Total RNA was extracted from normal and tumor plasma cells using miRNEasy Mini Kit (Qiagen, Valencia, USA) following manufacturer's protocol. The RNA integrity was assessed using Agilent 2100 Bioanalyzer (Agilent, Palo Alto, CA).
Label
biotin
Label protocol
Labelling and hybridizations were performed according to protocols from Affymetrix. Briefly, 100-300 ng of total RNA were amplified and labeled using the WT Sense Target labelling and control reagents kit (Affymetrix Inc., Santa Clara, CA, USA)
Hybridization protocol
Labeled RNA was hybridized to Human Gene 1.0 ST Array
Scan protocol
Washing and scanning were performed using GeneChip System of Affymetrix (GeneChip Hybridization Oven 640, GeneChip Fluidics Station 450 and GeneChip Scanner 7G).
Description
MM - Normal FISH - p694
Data processing
Expression value for each probe set was calculated using RMAExpress program that uses RMA (Robust Multi-Array Average) algorithm.
