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Status |
Public on Jul 08, 2020 |
Title |
Tet KO CD93+ B cells-3 (S13222) |
Sample type |
SRA |
|
|
Source name |
B cells
|
Organism |
Mus musculus |
Characteristics |
cell type: Primary B cells tissue: Freshly isolated from spleen strain: C57BL/6 genotype: Tet2/Tet3-deficient cell markers: CD93+
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was isolated using RNeasy micro kit (Qiagen) DNA library preparation for RNA-Seq analysis using a SureSelect Strand-Specific RNA Library Prep Kit for Illumina Multiplexed Sequencing (Agilent Technologies).
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|
|
Library strategy |
RNA-Seq |
Library source |
transcriptomic |
Library selection |
cDNA |
Instrument model |
Illumina HiSeq 1500 |
|
|
Description |
RNAseq_B_cell_3_subpopulations.csv
|
Data processing |
Read alignment: Bowtie2 v2.1.0 and TopHat2 v2.0.8 with default settings expression level: Cufflinks v2.1.1 with default settings FPKM (Fragments per Kirobase per Million reads) were calculated. Genome_build: mm9 Supplementary_files_format_and_content: CSV files containing FPKM values
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Submission date |
Sep 24, 2019 |
Last update date |
Jul 08, 2020 |
Contact name |
SHINYA TANAKA |
Organization name |
Kyushu University
|
Street address |
3-1-1, maidashi, Higashi-ku
|
City |
Fukuoka |
ZIP/Postal code |
812-8582 |
Country |
Japan |
|
|
Platform ID |
GPL18480 |
Series (1) |
GSE137914 |
Genome-wide mapping of gene expression in control and Tet2/Tet3-deficient B cell subsets |
|
Relations |
BioSample |
SAMN12828144 |
SRA |
SRX6894649 |