|
| Status |
Public on Jul 08, 2020 |
| Title |
Tet KO CD93-CD23+CD21 Int B cells-1 (S13200) |
| Sample type |
SRA |
| |
|
| Source name |
B cells
|
| Organism |
Mus musculus |
| Characteristics |
cell type: Primary B cells
tissue: Freshly isolated from spleen
strain: C57BL/6
genotype: Tet2/Tet3-deficient
cell markers: CD93-CD23+CD21 Int
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated using RNeasy micro kit (Qiagen)
DNA library preparation for RNA-Seq analysis using a SureSelect Strand-Specific RNA Library Prep Kit for Illumina Multiplexed Sequencing (Agilent Technologies).
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 1500 |
| |
|
| Description |
RNAseq_B_cell_3_subpopulations.csv
|
| Data processing |
Read alignment: Bowtie2 v2.1.0 and TopHat2 v2.0.8 with default settings
expression level: Cufflinks v2.1.1 with default settings
FPKM (Fragments per Kirobase per Million reads) were calculated.
Genome_build: mm9
Supplementary_files_format_and_content: CSV files containing FPKM values
|
| |
|
| Submission date |
Sep 24, 2019 |
| Last update date |
Jul 08, 2020 |
| Contact name |
SHINYA TANAKA |
| Organization name |
Kyushu University
|
| Street address |
3-1-1, maidashi, Higashi-ku
|
| City |
Fukuoka |
| ZIP/Postal code |
812-8582 |
| Country |
Japan |
| |
|
| Platform ID |
GPL18480 |
| Series (1) |
| GSE137914 |
Genome-wide mapping of gene expression in control and Tet2/Tet3-deficient B cell subsets |
|
| Relations |
| BioSample |
SAMN12828143 |
| SRA |
SRX6894650 |
