|
| Status |
Public on Sep 14, 2020 |
| Title |
erythroid cell obtained from CD34 culture - 48h - Rapamycine 2_Rapa_J10 |
| Sample type |
RNA |
| |
|
| Source name |
erythroid cell obtained from CD34 culture
|
| Organism |
Homo sapiens |
| Characteristics |
time: 48h
|
| Treatment protocol |
CX-5461 or vehicle were used on erythroid cell obtained from CD34 culture
|
| Growth protocol |
Human CD34+ progenitors were purified from cord blood units on MidiMacs system (Miltenyi Biotech, Bergisch Gladbach, Germany). Murine Extensively Self-Renewing Erythroblasts (ESREs) were derived from fetal livers. Cell differentiation was followed by cytological examination and by flow cytometry (see supplemental material).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted using the Kit QIAamp® RNA Blood MiniKit. RNA from ribosomal fractions was purified using Trizol (Invitrogen).
|
| Label |
biotin
|
| Label protocol |
Briefly, linear amplification of 20 ng of total RNA was performed using the Ovation Biotin RNA Amplification and Labelling System (NuGEN, San Carlos, CA).
|
| |
|
| Hybridization protocol |
cDNA was hybridized to HTA2.0 affymetrix microarrays following standard
|
| Scan protocol |
affymetrix microarrays following standard
|
| Description |
erythroid cell obtained from CD34 culture - 48h - Rapamycine
erythroid cell obtained from CD34 culture
|
| Data processing |
Raw expression values were normalized using Robust Multiarray Averaging (RMA)
|
| |
|
| Submission date |
Sep 24, 2019 |
| Last update date |
Sep 15, 2020 |
| Contact name |
Ismael Boussaid |
| E-mail(s) |
boussaid.ismael@gmail.com
|
| Phone |
+33618115659
|
| Organization name |
Inserm
|
| Lab |
Institut Cochin
|
| Street address |
22 Rue Mechain
|
| City |
Paris |
| ZIP/Postal code |
75014 |
| Country |
France |
| |
|
| Platform ID |
GPL22936 |
| Series (2) |
| GSE137951 |
p53 activation during ribosome biogenesis regulates normal erythroid differentiation. [expression] |
| GSE157210 |
p53 activation during ribosome biogenesis regulates normal erythroid differentiation. |
|
