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Sample GSM410165 Query DataSets for GSM410165
Status Public on Aug 27, 2009
Title small airways, smoker 264
Sample type RNA
 
Source name airway epithelial cells obtained by bronchoscopy and brushing
Organism Homo sapiens
Characteristics age: 39
sex: M
ethnic group: black
smoking status: smoker, 11 pack-years
Extracted molecule total RNA
Extraction protocol Trizol extraction and RNAeasy clean-up of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1-2 microg total RNA (Expression Analysis Technical Manual, 701022 Rev.2, Affymetrix).
 
Hybridization protocol Following fragmentation, 10 microg of cRNA were hybridized for 16 hr at 45C on GeneChip HG-U133 Plus 2.0. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned using the GeneChip Scanner 3000 7G.
Description small airways, smoker 264
Data processing The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
 
Submission date May 29, 2009
Last update date Aug 28, 2018
Contact name Yael Strulovici-Barel
E-mail(s) yas2003@med.cornell.edu
Organization name Weill Cornell Medical College
Department Department of Genetic Medicine
Lab Crystal
Street address 1300 York Avenue
City New York
State/province NY
ZIP/Postal code 10021
Country USA
 
Platform ID GPL570
Series (8)
GSE11784 Biologic Phenotyping of the Human Small Airway Epithelial Response to Cigarette Smoking
GSE13933 Trachea Epithelium as a “ Canary” for Cigarette Smoking-induced Biologic Phenotype of Small Airway Epithelium
GSE17905 Smoking-mediated Up-regulation of GAD67 Expression in the Human Airway Epithelium
Relations
Reanalyzed by GSE119087

Data table header descriptions
ID_REF
VALUE Signal
ABS_CALL indicating whether the transcript was present (P), absent (A), or marginal (M)
DETECTION P-VALUE

Data table
ID_REF VALUE ABS_CALL DETECTION P-VALUE
AFFX-BioB-5_at 1298.03 P 6.02111e-05
AFFX-BioB-M_at 2472.91 P 4.42873e-05
AFFX-BioB-3_at 1408.34 P 4.42873e-05
AFFX-BioC-5_at 4648.76 P 5.16732e-05
AFFX-BioC-3_at 6513.9 P 4.42873e-05
AFFX-BioDn-5_at 9987.32 P 4.42873e-05
AFFX-BioDn-3_at 16086.3 P 4.42873e-05
AFFX-CreX-5_at 64880.7 P 5.16732e-05
AFFX-CreX-3_at 67681.5 P 4.42873e-05
AFFX-DapX-5_at 47.4224 A 0.156732
AFFX-DapX-M_at 23.754 A 0.425962
AFFX-DapX-3_at 4.68954 A 0.949771
AFFX-LysX-5_at 7.33361 A 0.5
AFFX-LysX-M_at 43.9085 A 0.559354
AFFX-LysX-3_at 9.31678 A 0.574038
AFFX-PheX-5_at 27.9308 A 0.382599
AFFX-PheX-M_at 5.11113 A 0.957038
AFFX-PheX-3_at 24.5828 A 0.51489
AFFX-ThrX-5_at 37.5498 A 0.195246
AFFX-ThrX-M_at 20.0809 A 0.455409

Total number of rows: 54675

Table truncated, full table size 1640 Kbytes.




Supplementary file Size Download File type/resource
GSM410165.CEL.gz 7.9 Mb (ftp)(http) CEL
GSM410165.CHP.gz 13.0 Mb (ftp)(http) CHP
Processed data included within Sample table
Processed data provided as supplementary file

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