|
| Status |
Public on Oct 04, 2019 |
| Title |
WT_Hippocampus_rep3 |
| Sample type |
RNA |
| |
|
| Source name |
brain
|
| Organism |
Mus musculus |
| Characteristics |
genotype/variation: wild type
strain: C57BL/6
Sex: male
age: 6-month-old
|
| Treatment protocol |
Mice were deeply anesthetized with pentobarbital sodium and then hippocampus were rapidly dissected from the fresh brains.
|
| Growth protocol |
All mice were raised in Pathogen-free room and the room was maintained at a constant temperature of 23±1℃ with a 12 h light/dark cycle. Food and water were available ad libitum in the home cages.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
The hippocampus were isolated and homogenized using a Tissue homogenizer, and the RNA was extracted and purified using RNeasy Mini Kit.
|
| Label |
Cy3
|
| Label protocol |
Total RNA (100ng) from each sample (3 samples/group) was labeled using the Agilent miRNA Complete Labeling and Hybridization kit.
|
| |
|
| Hybridization protocol |
Each sample was resuspended in 17ml nuclease free water. A master hybridization master mix was made consisting of diluted Hyb Spike-In controls, Agilent 10X blocking agent and Agilent Hi-RPM hybridization buffer. Aliquots of the master mix were added to each resuspended sample to a final volume of 45ml. The hybridization mix was incubated for 5 min at 1000C and then transferred to an ice/water bath to be incubated for 5 min. The samples were then briefly centrifuged to collect any condensation from the tube caps. The entire amount for each sample was dispensed into the center of a gasket well of an Agilent Mouse miRNA Release 21.0, 8x60K microarray slide (8 samples/slide). Once all samples were applied to the gasket slide, the chamber cover was clamped in place. The assembled slide cassette was placed into the hybridization oven and hybridized for 20 hrs at 550C at 20 rpm.
|
| Scan protocol |
The array was scanned using Agilent Scan Control vA.8.5.1 with the AgilentG3_miRNA protocol at a resolution of 5mm.
|
| Data processing |
miRNAs with Absent calls across all samples were filtered out. Quantile normalization was used to normalize the remaining miRNAs.
|
| |
|
| Submission date |
Oct 03, 2019 |
| Last update date |
Oct 04, 2019 |
| Contact name |
XIAOLEI ZHU |
| E-mail(s) |
zhuquelee@gmail.com
|
| Phone |
02583106666
|
| Organization name |
Nanjing Drum Tower Hospital
|
| Department |
Neurology
|
| Street address |
321 ZHONGSHAN ROAD
|
| City |
NANJING |
| State/province |
JIANGSU |
| ZIP/Postal code |
210000 |
| Country |
China |
| |
|
| Platform ID |
GPL21439 |
| Series (1) |
| GSE138382 |
miRNA profile in the hippocampus of APP/PS1 transgenic mice |
|
