|
Status |
Public on Nov 18, 2010 |
Title |
2 IL-4 macrophage+Rosigl., 24h |
Sample type |
RNA |
|
|
Source name |
Macrophages treated with interleukin-4 and Rosiglitazone for 24h
|
Organism |
Homo sapiens |
Characteristics |
donor: 2 tissue: macrophage activation: IL-4 treatment: Rosiglitazone time: 24h
|
Treatment protocol |
Cell were treated after plating into 6-well cell culture plates with the indicated cytokines (100 ug/ml IL-4, or 100 ug/ml IFNg+50 ug/ml TNF. Simultaneously, vehicle (DMSO:ethanol) or 1 uM Rosiglitazone was added.
|
Growth protocol |
Isolated monocytes were cultured in RPMI+10% FCS supplemented with glutamine, penicillin and streptomycin.
|
Extracted molecule |
total RNA |
Extraction protocol |
Qiagen extraction of total RNA was performed according to the manufacturer's instructions.
|
Label |
biotin
|
Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
|
|
Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized on Human Genome U133 Plus 2 Affymetrix Array according to Affymetric standardized protocol.
|
Scan protocol |
Microarray were scanned canned according to Affymetric standardized protocol.
|
Description |
Gene expression data from macrophages treated with interleukin-4 and Rosiglitazone for 24h
|
Data processing |
The data were generated with GCOS, and cel files were analyzed in GeneSpring 7.3 using GC-RMA.
|
|
|
Submission date |
Jun 02, 2009 |
Last update date |
Sep 01, 2016 |
Contact name |
Attila Szanto |
E-mail(s) |
attila.szanto@gmail.com
|
Organization name |
Massachusetts General Hospital
|
Department |
Molecular Biology
|
Lab |
Lee Lab
|
Street address |
185 Cambridge St.
|
City |
Boston |
State/province |
MA |
ZIP/Postal code |
02446 |
Country |
USA |
|
|
Platform ID |
GPL570 |
Series (2) |
GSE16385 |
Expression data from human macrophages |
GSE16387 |
Licensing of PPARg-regulated gene expression by IL-4-induced alternative macrophage activation |
|
Relations |
Reanalyzed by |
GSE86362 |