|
| Status |
Public on Nov 18, 2010 |
| Title |
3 IFNg+TNF macrophage, 72h |
| Sample type |
RNA |
| |
|
| Source name |
Macrophages treated with IFNg+TNF and vehicle for 72h
|
| Organism |
Homo sapiens |
| Characteristics |
donor: 3
tissue: macrophage
activation: IFNg+TNF
treatment: Control
time: 72h
|
| Treatment protocol |
Cell were treated after plating into 6-well cell culture plates with the indicated cytokines (100 ug/ml IL-4, or 100 ug/ml IFNg+50 ug/ml TNF. Simultaneously, vehicle (DMSO:ethanol) or 1 uM Rosiglitazone was added.
|
| Growth protocol |
Isolated monocytes were cultured in RPMI+10% FCS supplemented with glutamine, penicillin and streptomycin.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Qiagen extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized on Human Genome U133 Plus 2 Affymetrix Array according to Affymetric standardized protocol.
|
| Scan protocol |
Microarray were scanned canned according to Affymetric standardized protocol.
|
| Description |
Gene expression data from macrophages treated with IFNg+TNF and vehicle for 72h
|
| Data processing |
The data were generated with GCOS, and cel files were analyzed in GeneSpring 7.3 using GC-RMA.
|
| |
|
| Submission date |
Jun 02, 2009 |
| Last update date |
Sep 01, 2016 |
| Contact name |
Attila Szanto |
| E-mail(s) |
attila.szanto@gmail.com
|
| Organization name |
Massachusetts General Hospital
|
| Department |
Molecular Biology
|
| Lab |
Lee Lab
|
| Street address |
185 Cambridge St.
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02446 |
| Country |
USA |
| |
|
| Platform ID |
GPL570 |
| Series (2) |
| GSE16385 |
Expression data from human macrophages |
| GSE16387 |
Licensing of PPARg-regulated gene expression by IL-4-induced alternative macrophage activation |
|
| Relations |
| Reanalyzed by |
GSE86362 |
