|
Status |
Public on Oct 12, 2019 |
Title |
C4-2B-TaxR_Vehicle_48hrs |
Sample type |
SRA |
|
|
Source name |
C4-2B-TaxR cells treated with vehicle for 48 hrs
|
Organism |
Homo sapiens |
Characteristics |
cell line: C4-2B-TaxR prostate cancer cell line with resistance to taxels treatment: vehicle
|
Treatment protocol |
C4-2B-TaxR cells were seeded into 6 well plate and, next day, cells were treated with vehicle, 10 nM docetaxel, and 2 microM SR2211 for 48 hours.
|
Growth protocol |
C4-2B-TaxR cells were cultured in RPMI supplemented with 10% FBS and 5 nM docetaxel. Cells were grown at 37℃ in 5% CO2 incubators.
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was extracted using the TRIzol Reagent (Invitrogen) from the treated cells. RNA-seq libraries from 1 µg total RNA were prepared using Illumina Tru-Seq RNA Sample Prep Kit, according to the manufacture's instruction.
|
|
|
Library strategy |
RNA-Seq |
Library source |
transcriptomic |
Library selection |
cDNA |
Instrument model |
Illumina HiSeq 2000 |
|
|
Data processing |
Sequencing was perforemd on an Illumina HiSeq 2000 sequencer. The FASTQ-formatted sequence data were analyzed using a standard BWA-Bowtie-Cufflinks workflow. Sequence reads were mapped to GRCh37/hg19 assembly with BWA and Biotie software. The Cufflinks package was used for transcripts assembly, quantification of normalized gene and isoform expression, and analysis of different expression. Genome_build: hg19 Supplementary_files_format_and_content: abundance measurements
|
|
|
Submission date |
Oct 11, 2019 |
Last update date |
Oct 15, 2019 |
Contact name |
Hongwu Chen |
E-mail(s) |
hwzchen@ucdavis.edu
|
Phone |
9167347743
|
Organization name |
UC Davis
|
Department |
Biochem & Mol Med
|
Street address |
2nd Ave, UCDMC Res III
|
City |
Sacramento |
State/province |
California |
ZIP/Postal code |
95817 |
Country |
USA |
|
|
Platform ID |
GPL11154 |
Series (1) |
GSE138751 |
Therapeutic targeting MDR1 expression by RORγ antagonists resensitizes cross-resistant CRPC to taxane via coordinated induction of cell death programs |
|
Relations |
BioSample |
SAMN13017400 |
SRA |
SRX6978655 |