|
| Status |
Public on Dec 22, 2020 |
| Title |
SAMPLE 14_KO-CYP2C70_UDCA Feeding 0.1% |
| Sample type |
RNA |
| |
|
| Source name |
KO-CYP2C70_UDCA Feeding 0.1%_Liver
|
| Organism |
Mus musculus |
| Characteristics |
strain background: C57BL/6J
genotype/variation: KO-CYP2C70
Sex: Females
age: 12wks
diet: UDCA Feeding 0.1%
tissue: liver
|
| Treatment protocol |
Total RNA was prepared form Liver tissue of 12 wk old Female mice fed an 0.1% UDCA chow diet for 7wks , starting at the age of 5wks or a regular Chow dieet.
|
| Growth protocol |
Mice were housed in a temperature-controlled room (22–24°C) and 12-hour light/12-hour dark cycles.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted using nucleosol with cleanup on Mn RNA columns and reverse transcribed using the High Capacity cDNA Reverse Transcription kit (Applied Biosystems, USA). Total RNA was checked for quantity and quality using the Agilent 2100 Bioanalyzer (Agilent Biotechnologies)
|
| Label |
Biotin
|
| Label protocol |
RNA were processed using Affymetrix GeneChip® WT PLUS Reagent Kit
|
| |
|
| Hybridization protocol |
Affymetrix GeneChip Hybridization Oven 640 for 16h at 45°C and 60 rpm on MoGene-2_0-st Chip. GeneChips were washed and stained in the Affymetrix Fluidics
|
| Scan protocol |
Affymetrix GeneChip Scanner 7G and Affymetrix GeneChip Command Console software (AGCC)
|
| Description |
RMA - PLM (unlogged scale): 14-m15_(MoGene-2_0-st).CEL (KO_UDCA)
|
| Data processing |
The data where normalized using RMA (PLM) normalisation strategies using the MADMAX analysis pipeline pmid:21778530
|
| |
|
| Submission date |
Oct 11, 2019 |
| Last update date |
Dec 22, 2020 |
| Contact name |
vincent bloks |
| Organization name |
UMCG
|
| Department |
Pediatrics
|
| Lab |
Pediatrics
|
| Street address |
Hanzeplein1
|
| City |
Groningen |
| ZIP/Postal code |
9713GZ |
| Country |
Netherlands |
| |
|
| Platform ID |
GPL20710 |
| Series (1) |
| GSE138779 |
Cholangiopathy and biliary fibrosis in Cyp2c70-deficient mice are fully reversed by ursodeoxycholic acid |
|
