|
| Status |
Public on Oct 15, 2019 |
| Title |
control embryos 2 |
| Sample type |
RNA |
| |
|
| Source name |
control embryos
|
| Organism |
Xenopus laevis |
| Characteristics |
tissue: gastrula dorsal half
genotype: control MO
age: st. 10.5
|
| Treatment protocol |
2 dorsal injection of 10 ng Apcdd1 morpholino oligonucleotides at 4 cell stage
|
| Growth protocol |
0.1X MMR until stage 10.5, at 16 deg. C
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
|
| |
|
| Submission date |
Oct 14, 2019 |
| Last update date |
Oct 15, 2019 |
| Contact name |
Alin Vonica |
| E-mail(s) |
avonica4@naz.edu
|
| Organization name |
The Nazareth College
|
| Department |
Biology
|
| Street address |
4245 East Avenue
|
| City |
Rochester |
| State/province |
NY |
| ZIP/Postal code |
14618 |
| Country |
USA |
| |
|
| Platform ID |
GPL10756 |
| Series (1) |
| GSE138834 |
Gene expression in dorsal halves of Apcdd1-depleted Xenopus laevis embryos |
|
