|
| Status |
Public on Oct 01, 2020 |
| Title |
KL Null_2 |
| Sample type |
RNA |
| |
|
| Source name |
kidney
|
| Organism |
Mus musculus |
| Characteristics |
strain: 129/SvEv/C57BL/6
tissue: kidney
genotype: KL null
treatment: injected with 100ul saline vehicle
gender: Female
|
| Treatment protocol |
KL-null mice (20) and wild type littermates (N=4-6) were maintained on standard diet for 5-8 weeks and given one intraperitoneal injection of 500 ng/g body weight recombinant HBEGF (R&D System), 500 ng/g body weight recombinant FGF23 (R&D System) or 100 µl saline vehicle i.p., then sacrificed after one hour.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol
|
| Label |
biotin
|
| Label protocol |
The RNA was labeled following the standard WT protocol (GeneChip® Whole Transcript (WT) Sense Target Labeling Assay, rev. 5, www.affymetrix.com).
|
| |
|
| Hybridization protocol |
Labeled RNA was hybridized to Affymetrix Mouse Gene 1.0 ST arrays for 17 hours then washed using the Affymetrix standard protocol (GeneChip® Whole Transcript (WT) Sense Target Labeling Assay, rev. 5)
|
| Scan protocol |
Arrays were scanned using
|
| Data processing |
Data were imported into Partek Genomics Suite version 6.2 (Partek, Inc. St. Louis, Mo). Robust Multichip Average signals (RMA) (21) were generated for the core probe sets using the RMA background correction, Quantile normalization and summarization by Median Polish using Partek Genomics Suite. Summarized signals for each probe set were log2 transformed.
|
| |
|
| Submission date |
Oct 16, 2019 |
| Last update date |
Oct 02, 2020 |
| Contact name |
Kenneth E. White |
| Organization name |
Indiana University School of Medicine
|
| Street address |
635 Barnhill Dr, MS 5012
|
| City |
Indianapolis |
| State/province |
IN |
| ZIP/Postal code |
46202 |
| Country |
USA |
| |
|
| Platform ID |
GPL6246 |
| Series (1) |
| GSE138992 |
Targeting Fibroblast growth factor-23 (FGF23) responsive pathways in the control of renal phosphate and vitamin D metabolism |
|
