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| Status |
Public on Oct 19, 2019 |
| Title |
Glucose feed (SP) |
| Sample type |
SRA |
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| Source name |
Sponge biomass
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| Organism |
wastewater metagenome |
| Characteristics |
sample type: Sponge biomass
mbr system: Biofilm anoxic-oxic
carbon source: Glucose
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| Extracted molecule |
total RNA |
| Extraction protocol |
Fast-DNA SPIN kit for soil (MP Biomedicals, Solon, OH, USA).
RNA libraries were prepared for sequencing using standard Illumina protocols.
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| Library strategy |
OTHER |
| Library source |
transcriptomic |
| Library selection |
other |
| Instrument model |
Illumina MiSeq |
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| Description |
Bacteria
processed data file: Classification.txt
processed data file: Glucose_feed_SP.report.pdf
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| Data processing |
Sequencing was carried out by using Illumina MiSeq sequencing on an Illumina MiSeq device (Illumina Inc., San Diego, CA, USA) with 600 cycles (300 cycles for each paired read and 12 cycles for the barcode sequence) according to the manufacturer’s instructions.
Sequence analysis was conducted using the 16S-based metagenomics workflow of MiSeq Reporter version 2.6.2.3 (Illumina).
Samples were gathered into a single library for sequencing on Illumina MiSeq sequencing system which generated paired 300 bp reads.
Sequences were then demultiplexed based on index sequences.
Genome_build: V3 and V4 regions of the 16S rRNA gene.
Supplementary_files_format_and_content: Classification.txt file includes Sample 1 Number 1SS (Glucose feed (SS)), Sample 2 Number 1SP (Glucose feed (SP)), Sample 3 number 2_1SS (Acetate feed (SS)), Sample 4 number 2_1SP (Acetate feed (SP)), Sample 5 number 7SS (Propionate feed (SS)) and Sample 6 number 8Ano (Propinate feed (SP)).
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| Submission date |
Oct 18, 2019 |
| Last update date |
Oct 21, 2019 |
| Contact name |
Dome Adoonsook |
| E-mail(s) |
dome_fb@hotmail.com
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| Phone |
0899562526
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| Organization name |
Dome
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| Street address |
Suthep
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| City |
muang |
| State/province |
ChiangMai |
| ZIP/Postal code |
50100 |
| Country |
Thailand |
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| Platform ID |
GPL27052 |
| Series (1) |
| GSE139113 |
Comparison of microbial communities with different carbon source types |
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| Relations |
| BioSample |
SAMN13060797 |
| SRA |
SRX7023960 |
| Supplementary data files not provided |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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