|
| Status |
Public on Aug 24, 2021 |
| Title |
siCtrl_replicate2 |
| Sample type |
RNA |
| |
|
| Source name |
Human mature adipocytes, de-novo differentiated in cell culture from isolated stromal vascual fraction of lipoaspirates; control sample, transfected with control siRNA, replicate 2
|
| Organism |
Homo sapiens |
| Characteristics |
sirna: control siRNA
|
| Treatment protocol |
Treated Human mature adipocytes, de-novo differentiated from stromal vascular fraction (SVF) derived from lipoaspirates from 2 different donors. One pool of adipocytes was transfected with a non-targeting siRNA (siCtrl) and the other pool was transfected with a siRNA targeting human LMO3 gene.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Rneasy mini kit (Qiagen)
|
| Label |
Biotin
|
| Label protocol |
100ng of total RNA were amplified and labeled using the Ambion Whole Transcript Expression Kit (Catalog Number 4425209, Ambion, Austin, TX) and Affymetix Genechip Whole Transcript Terminal Labeling Kit (Catalog Number 900671, Affymetrix, Santa Clara, CA)
|
| |
|
| Hybridization protocol |
Affymetrix GeneChip® Human Gene 1.0 ST arrays were washed, stained and scanned according to the protocol described in GeneChip® WT Terminal Labeling and Hybridization User Manual (Fluidics Protocol FS450_0007)
|
| Scan protocol |
Scanning was done on an Affymetrix GeneChip Scanner 3000 7G, software AGCC 3.1.1
|
| Data processing |
RMA (Robust Multiarray average) Signal extraction using Affymetrix Expression Console Version 1.1
|
| |
|
| Submission date |
Oct 21, 2019 |
| Last update date |
Aug 24, 2021 |
| Contact name |
Markus Jeitler |
| E-mail(s) |
markus.jeitler@meduniwien.ac.at
|
| Organization name |
Medical University Vienna
|
| Street address |
Lazarettgasse 14
|
| City |
Vienna |
| ZIP/Postal code |
A-1090 |
| Country |
Austria |
| |
|
| Platform ID |
GPL6244 |
| Series (2) |
| GSE139155 |
LMO3 reprogramms viseral adipocyte metabolism during obesity [Human] |
| GSE139163 |
LMO3 reprogramms viseral adipocyte metabolism during obesity |
|
