|
| Status |
Public on Jan 02, 2020 |
| Title |
RNA-Seq of Zeugodacus cucurbitae female 1 [F1_2] |
| Sample type |
SRA |
| |
|
| Source name |
female 1-d-old
|
| Organism |
Zeugodacus cucurbitae |
| Characteristics |
tissue: whole body
developmental stage: Early female adult
replicate: Biological replicate 2
|
| Treatment protocol |
NA
|
| Growth protocol |
Z. cucurbitae were reared at 26 – 27ºC with 70% relative humidity and a photoperiod of 14:10 h light: dark.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using TRIZol reagent
RNA libraries were prepared for sequencing using standard Illumina protocols
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
HiSeq X Ten |
| |
|
| Data processing |
Illumina CASAVA1.7 software used for base calling
The quality of the raw data was evaluated by fastx_toolkit_0.0.14 for the low-complexity or low-quality sequence
Raw reads were quanlity controled by SeqPrep and Sickle software
Clean reads were aligned to the reference genome using HISAT2 2.1.0
Gene expression analysis was conducted based on the sequenced reads using RNA-Seq with the Expectation-Maximization (RSEM, v1.3.1) method
Genome_build: https://www.ncbi.nlm.nih.gov/assembly/GCF_000806345.1/
Supplementary_files_format_and_content: tpm
|
| |
|
| Submission date |
Oct 28, 2019 |
| Last update date |
Jan 02, 2020 |
| Contact name |
Dong Wei |
| E-mail(s) |
dong_wei1988@yahoo.com
|
| Organization name |
Southwest University
|
| Street address |
Tiansheng
|
| City |
Beibei |
| State/province |
Chongqing |
| ZIP/Postal code |
400716 |
| Country |
China |
| |
|
| Platform ID |
GPL27683 |
| Series (1) |
| GSE139488 |
Transcriptomes of different developmental stages of Zeugodacus cucurbitae |
|
| Relations |
| BioSample |
SAMN12631764 |
| SRA |
SRX6792367 |
