|
Status |
Public on Oct 31, 2019 |
Title |
tail-PS-vc4 |
Sample type |
RNA |
|
|
Source name |
Tail clip, Transferred, Pond-Stream
|
Organism |
Salamandra salamandra |
Characteristics |
sampleID: vc4 tissue: tail clip treatment: PS origin: KoV destination: KoGc age: larva location: Kottenforst, Germany
|
Treatment protocol |
At the end of the experiment, larvae were tail-clipped. Tail-clips were stored in RNA-later and kept at −20 °C, until further analysis.
|
Growth protocol |
Larvae were captured and placed in the containers on 11 May 2015 for a reciprocal transplant experiment between ponds and streams, the experiment lasted 14 days.
|
Extracted molecule |
total RNA |
Extraction protocol |
The total RNA was extracted using the Thermo Fisher TRIzol protocol
|
Label |
Cy3
|
Label protocol |
Cyanine-3 (Cy3)
|
|
|
Hybridization protocol |
Agilent Low Input Quick Amp Labelling kit for ‘One-Color Microarray-Based Gene Expression Analysis’
|
Scan protocol |
Agilent DNA Microarray Scanner (type C)
|
Description |
Gene expression from tail clip from a Pond-originated larvae translocated/kept in a Stream
|
Data processing |
feature extraction software (Agilent)
|
|
|
Submission date |
Oct 30, 2019 |
Last update date |
Oct 31, 2019 |
Contact name |
Joana Sabino Pinto |
E-mail(s) |
joanasabinopinto@gmail.com
|
Organization name |
Technische Universität Braunschweig, Zoologisches Institute
|
Street address |
Mendelsohnstrasse 4
|
City |
Braunschweig |
ZIP/Postal code |
38106 |
Country |
Germany |
|
|
Platform ID |
GPL23661 |
Series (1) |
GSE139590 |
The role of plasticity and adaptation on in the incipient speciation of a fire salamander population |
|