|
| Status |
Public on Oct 31, 2019 |
| Title |
tail-PP-ee4 |
| Sample type |
RNA |
| |
|
| Source name |
Tail clip, Transferred, Pond-Pond
|
| Organism |
Salamandra salamandra |
| Characteristics |
sampleID: ee4
tissue: tail clip
treatment: PP
origin: KoE
destination: KoE
age: larva
location: Kottenforst, Germany
|
| Treatment protocol |
At the end of the experiment, larvae were tail-clipped. Tail-clips were stored in RNA-later and kept at −20 °C, until further analysis.
|
| Growth protocol |
Larvae were captured and placed in the containers on 11 May 2015 for a reciprocal transplant experiment between ponds and streams, the experiment lasted 14 days.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
The total RNA was extracted using the Thermo Fisher TRIzol protocol
|
| Label |
Cy3
|
| Label protocol |
Cyanine-3 (Cy3)
|
| |
|
| Hybridization protocol |
Agilent Low Input Quick Amp Labelling kit for ‘One-Color Microarray-Based Gene Expression Analysis’
|
| Scan protocol |
Agilent DNA Microarray Scanner (type C)
|
| Description |
Gene expression from tail clip from a Pond-originated larvae translocated/kept in a Pond
|
| Data processing |
feature extraction software (Agilent)
|
| |
|
| Submission date |
Oct 30, 2019 |
| Last update date |
Oct 31, 2019 |
| Contact name |
Joana Sabino Pinto |
| E-mail(s) |
joanasabinopinto@gmail.com
|
| Organization name |
Technische Universität Braunschweig, Zoologisches Institute
|
| Street address |
Mendelsohnstrasse 4
|
| City |
Braunschweig |
| ZIP/Postal code |
38106 |
| Country |
Germany |
| |
|
| Platform ID |
GPL23661 |
| Series (1) |
| GSE139590 |
The role of plasticity and adaptation on in the incipient speciation of a fire salamander population |
|
