|
| Status |
Public on Jul 07, 2020 |
| Title |
PTID8_Enrollment_PBMC |
| Sample type |
RNA |
| |
|
| Source name |
PBMC from PTID8 at Enrollment
|
| Organism |
Homo sapiens |
| Characteristics |
ptid: PTID8
visit: Enrollment
tissue: PBMC
rin: 9.4
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using the Qiagen RNeasy mini kit
|
| Label |
Cy3
|
| Label protocol |
Labeling was performed using 275 ng total RNA and the Life Technologies/Ambion Illumina TotalPrep RNA Amplification kit
|
| |
|
| Hybridization protocol |
standard as recommended by illumina
|
| Scan protocol |
standard Illumina protocol using Illumina iScan system
|
| Data processing |
The data was preprocessed by Bioconductor lumi package (version 2.34.0). It was vst transformed and rsn normalized. Only probes with detection threshold < 0.05 in at least 7 samples were included in the analysis.
|
| |
|
| Submission date |
Oct 30, 2019 |
| Last update date |
Jul 07, 2020 |
| Contact name |
Sean Hughes |
| E-mail(s) |
smhughes@uw.edu
|
| Organization name |
University of Washington
|
| Street address |
850 Republican St
|
| City |
Seattle |
| State/province |
WA |
| ZIP/Postal code |
98109 |
| Country |
USA |
| |
|
| Platform ID |
GPL10558 |
| Series (2) |
| GSE139609 |
Effect of pre-exposure prophylaxis with tenofovir disoproxil fumarate and emtricitabine on gene expression in PBMC |
| GSE139611 |
Effect of pre-exposure prophylaxis with tenofovir disoproxil fumarate and emtricitabine on gene expression |
|
