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Status |
Public on Jul 10, 2022 |
Title |
HCHF_2years_SAMPLE 84 |
Sample type |
RNA |
|
|
Source name |
Baboon Frozen Liver
|
Organism |
Papio hamadryas |
Characteristics |
gender: Male timepoint: 2 years diet: HCHF diet tissue: liver sample number: 84
|
Treatment protocol |
Biotinylated cRNA were prepared with the Amp kit for Illumina arrays
|
Extracted molecule |
total RNA |
Extraction protocol |
RNA was extracted from the homogenate and impurities removed using the RNeasy MinElute Kit
|
Label |
biotin
|
Label protocol |
cRNA was synthesized and biotin labeled using Illumina TotalPrep RNA Amplification Kit.
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|
|
Hybridization protocol |
Standard Illumina hybridization protocol
|
Scan protocol |
Standard Illumina hybridization protocol
|
Description |
IL_BPP2yc_252_17165_HFHC_2yr_liv.AVG_Signal
|
Data processing |
data for all samples were pre-processed identically for background correction and normalization with Illumina Data filtering: Only those transcripts whose detection levels equaled or exceeded 0.2 (of the detection p-value 0.95) in greater or equal to 50% of the total sample were filtered. The filtered transcript data were quantile normalized and log2 transformed. normalized data file includes quantile normalized and log2 transformed with GUI R
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Submission date |
Nov 05, 2019 |
Last update date |
Jul 10, 2022 |
Contact name |
Laura A Cox |
Organization name |
Wake Forest School of Medicine
|
Department |
Internal medicine-Section of Molecular Medicine
|
Lab |
Cox Lab
|
Street address |
Medical Center Boulevard
|
City |
Winston-Salem |
State/province |
NC |
ZIP/Postal code |
27157 |
Country |
USA |
|
|
Platform ID |
GPL10558 |
Series (2) |
GSE139979 |
Hepatic transcript modules are predictive of atherosclerotic lesion burden after a 2-year high cholesterol, high fat diet challenge in baboons [2 years HCHF] |
GSE139981 |
Hepatic transcript modules are predictive of atherosclerotic lesion burden after a 2-year high cholesterol, high fat diet challenge in baboons |
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