|
| Status |
Public on Jul 10, 2022 |
| Title |
HCHF_2years_SAMPLE 103 |
| Sample type |
RNA |
| |
|
| Source name |
Baboon Frozen Liver
|
| Organism |
Papio hamadryas |
| Characteristics |
gender: Female
timepoint: 2 years
diet: HCHF diet
tissue: liver
sample number: 103
|
| Treatment protocol |
Biotinylated cRNA were prepared with the Amp kit for Illumina arrays
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted from the homogenate and impurities removed using the RNeasy MinElute Kit
|
| Label |
biotin
|
| Label protocol |
cRNA was synthesized and biotin labeled using Illumina TotalPrep RNA Amplification Kit.
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina hybridization protocol
|
| Description |
IL_BPP2yc_309_16761_HFHC_2yr_liv.AVG_Signal
|
| Data processing |
data for all samples were pre-processed identically for background correction and normalization with Illumina
Data filtering: Only those transcripts whose detection levels equaled or exceeded 0.2 (of the detection p-value 0.95) in greater or equal to 50% of the total sample were filtered. The filtered transcript data were quantile normalized and log2 transformed.
normalized data file includes quantile normalized and log2 transformed with GUI R
|
| |
|
| Submission date |
Nov 05, 2019 |
| Last update date |
Jul 10, 2022 |
| Contact name |
Laura A Cox |
| Organization name |
Wake Forest School of Medicine
|
| Department |
Internal medicine-Section of Molecular Medicine
|
| Lab |
Cox Lab
|
| Street address |
Medical Center Boulevard
|
| City |
Winston-Salem |
| State/province |
NC |
| ZIP/Postal code |
27157 |
| Country |
USA |
| |
|
| Platform ID |
GPL10558 |
| Series (2) |
| GSE139979 |
Hepatic transcript modules are predictive of atherosclerotic lesion burden after a 2-year high cholesterol, high fat diet challenge in baboons [2 years HCHF] |
| GSE139981 |
Hepatic transcript modules are predictive of atherosclerotic lesion burden after a 2-year high cholesterol, high fat diet challenge in baboons |
|
