|
| Status |
Public on Dec 12, 2019 |
| Title |
CHO-K1 NoPUT Day3, biological rep2 |
| Sample type |
RNA |
| |
|
| Source name |
CHO-K1 cultures in media without Putrescine
|
| Organism |
Cricetulus griseus |
| Characteristics |
cell line: CHO-K1
cell type: Chinese Hamster Ovary cell line
culture type: Suspension, 50 ml at 37 C, 170 rpm, 5 % CO2 and 80 % humidity
treatment: starved for putrescine (growing in media without putrescine) for 3 days
|
| Treatment protocol |
Triplicate CHO-K1 cultures were seeded at 200,000 cells/ml in 50 ml in SFM-F10 media (Capella Roca et al. 2019) supplemented with 1 mg/l putrescine (+PUT) and without putrescine (NoPUT) for three days
|
| Growth protocol |
CHO-K1 suspension cells in 50 ml working volume in 250ml flasks (Corning) and maintained in an ISF1-X (Climo Shaker) Kuhner incubator
|
| Extracted molecule |
total RNA |
| Extraction protocol |
A total of 4,000,000 cells were collected from the triplicate flasks grown with putrescine (+PUT) and without putrescine (NoPUT), and RNA was extracted using 1ml Trizol according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to GeneChip™ WT PLUS Reagent Kit Manual Target Preparation for GeneChip™Whole Transcript (WT) Expression Arrays from 300 ng total RNA.
|
| |
|
| Hybridization protocol |
Following fragmentation, 3.5 ug of ssDNA were hybridized for 16 h, 60 rpm at 45 C on GeneChip CHO Gene 2.0 ST array. The GeneChips were washed and stained in the GenChip Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Affimetrix GeneChip scanner.
|
| Description |
F10NoPUT_N2_
|
| Data processing |
The data were analysed using the Transcriptome Analysis Console (TAC)
|
| |
|
| Submission date |
Nov 12, 2019 |
| Last update date |
Dec 13, 2019 |
| Contact name |
Berta Capella Roca |
| E-mail(s) |
berta.capellaroca2@mail.dcu.ie
|
| Organization name |
National Institute for Cellular Biotechnology
|
| Street address |
The National Institute for Cellular Biotechnology, Dublin City University, Glasnevin
|
| City |
Dublin |
| ZIP/Postal code |
Dublin 9 |
| Country |
Ireland |
| |
|
| Platform ID |
GPL24076 |
| Series (1) |
| GSE140223 |
Expression data from CHO-K1 cells in putrescine-deprived conditions |
|
