|
| Status |
Public on Dec 31, 2022 |
| Title |
EF_hESC_2 |
| Sample type |
RNA |
| |
|
| Source name |
human Embryonic Stem Cells expressing EWSFLI1
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: hESC
cell type: Embryonic Stem Cells
genotype/variation: ectopic EWSR1/FLI1 expression
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted following Sigma-Aldrich protocol
|
| Label |
biotin
|
| Label protocol |
100ng of total RNA from each sample was processed and labelled according to manual GeneChip WT PLUS Reagent kit (P/N 703174 Rev. 2, Affymetrix Inc., Santa Clara, CA, USA)
|
| |
|
| Hybridization protocol |
Sample was hybridized to Affymetrix Human Gene 2.0 ST array using an Affymetrix GeneChip Hybridization Oven 645
|
| Scan protocol |
GeneChip was scanned using the Affymetrix Expression Wash, Stain and Scan User Manual (P/N 702731 Rev. 3. Affymetrix Inc., Santa Clara, CA, USA) using Affymetrix GeneChip Scanner 3000 7G
|
| Data processing |
The data were analyzed using the RMA algorithm and then LIMMA was applied to calculate significant differential expression between samples
|
| |
|
| Submission date |
Nov 13, 2019 |
| Last update date |
Oct 19, 2023 |
| Contact name |
Júlia Perera-Bel |
| E-mail(s) |
mardata-bu@researchmar.net
|
| Organization name |
Hospital del Mar Research Institute
|
| Department |
MARData-BU
|
| Street address |
Doctor Aiguader, 88
|
| City |
Barcelona |
| ZIP/Postal code |
08003 |
| Country |
Spain |
| |
|
| Platform ID |
GPL16686 |
| Series (2) |
| GSE140328 |
Modeling the initiation of Ewing sarcoma tumorigenesis in human Embryonic Stem Cells [expression array] |
| GSE141889 |
Modeling the initiation of Ewing sarcoma tumorigenesis in human Embryonic Stem Cells |
|
