|
| Status |
Public on Jun 01, 2010 |
| Title |
HF3 vs. HF4 17722 |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
HF3
|
| Organism |
Mus musculus |
| Characteristics |
subject: HF3
tissue: epididymal fat
diet: high fat diet
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol
|
| Label |
Cy3
|
| Label protocol |
Ambion
|
| |
|
| Channel 2 |
| Source name |
HF4
|
| Organism |
Mus musculus |
| Characteristics |
subject: HF4
tissue: epididymal fat
diet: high fat diet
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol
|
| Label |
Cy5
|
| Label protocol |
Ambion
|
| |
|
| |
| Hybridization protocol |
CGH-Agilent-4rpm : 750.0 ng at 62 degree_C during 17 hours
|
| Scan protocol |
Scanned on an GenePix 4000B fluorescent scanner. Image intensity data were extracted with GenePix Pro 6.0 analysis software.
|
| Description |
HF3 vs HF4
Date:2006/10/10
|
| Data processing |
Interslide norm. : vsn, Intraslide norm. : none, Background norm. : none, Software used : rscript --> VALUE data obtained from log of processed Red signal/processed Green signal.
|
| |
|
| Submission date |
Jun 16, 2009 |
| Last update date |
Jun 23, 2009 |
| Contact name |
Kevin Lebrigand |
| Organization name |
IPMC/CNRS
|
| Lab |
Functional Genomics Platform of Nice-Sophia-Antipolis, France.
|
| Street address |
660 route des lucioles
|
| City |
Valbonne - Sophia-Antipolis |
| ZIP/Postal code |
06560 |
| Country |
France |
| |
|
| Platform ID |
GPL1476 |
| Series (1) |
| GSE16636 |
A human-like fat diet is sufficient to induce transgenerational obesity |
|
