|
| Status |
Public on May 24, 2010 |
| Title |
AWRIB421_versus_AWRIB515 |
| Sample type |
genomic |
| |
|
| Channel 1 |
| Source name |
AWRIB421
|
| Organism |
Oenococcus oeni |
| Characteristics |
strain: AWRIB421
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
phenol/chloroform DNA extraction
|
| Label |
Cy5
|
| Label protocol |
200ng DNA was random primer labeled using klenow (Bioprime) with amino-allyl dUTP and coupled to Amersham dye.
|
| |
|
| Channel 2 |
| Source name |
AWRIB515
|
| Organism |
Oenococcus oeni |
| Characteristics |
strain: AWRIB515
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
phenol/chloroform DNA extraction
|
| Label |
Cy3
|
| Label protocol |
200ng DNA was random primer labeled using klenow (Bioprime) with amino-allyl dUTP and coupled to Amersham dye.
|
| |
|
| |
| Hybridization protocol |
16hrs at 60°C in Agilent hybridization buffer using MAUI hybridization chamber. Washed using 2XSSC, 0.1% SDS for 10mins at 60°C, 0.1XSSC, 0.1%SDS for 19mins at RT, and four washes of 0.1XSSC for 1min at RT.
|
| Scan protocol |
Axon Genepix
|
| Description |
CGH anlysis of strain AWRIB421
|
| Data processing |
.gpr files were converted to .mev format using "express converter v1.8". Files were normalized using Midas v2.19 using Lowess normalization.
|
| |
|
| Submission date |
Jun 21, 2009 |
| Last update date |
May 24, 2010 |
| Contact name |
Anthony R Borneman |
| E-mail(s) |
anthony.borneman@awri.com.au
|
| Phone |
61 8 8303 6600
|
| Organization name |
The Australian Wine Research Institute
|
| Street address |
Paratoo Rd
|
| City |
Urrbrae |
| State/province |
South Australia |
| ZIP/Postal code |
5064 |
| Country |
Australia |
| |
|
| Platform ID |
GPL8742 |
| Series (1) |
| GSE16736 |
Oenococcus oeni CGH 11strains vs AWRIB515 |
|
