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| Status |
Public on Mar 06, 2020 |
| Title |
OV_18h_3 [miRNA-seq] |
| Sample type |
SRA |
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| Source name |
GSF cells infected by ORFV at 18h.p.i
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| Organism |
Capra hircus |
| Characteristics |
tissue source: skin
cell type: primary goat skin fibroblast (GSF) cells
treatment: GSF cells infected by ORFV at 18h.p.i
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| Extracted molecule |
total RNA |
| Extraction protocol |
Ambion mirVana miRNA Isolation Kit (Thermo Fisher Scientific)
TruSeq Small RNA Sample Prep Kits(Illumina, San Diego, USA)
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| Library strategy |
miRNA-Seq |
| Library source |
transcriptomic |
| Library selection |
size fractionation |
| Instrument model |
Illumina HiSeq 2500 |
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| Data processing |
Single-end sequencing on Illumina HiSeq 2500
ACGT101-miR (LC Sciences, Houston, Texas, USA) to remove adapter dimers, junk, low complexity, common RNA families (rRNA, tRNA, snRNA, snoRNA) and repeats.
unique sequences with length in 18~26 nucleotide were mapped to specific species precursors in miRBase 21.0 by BLAST search to identify known miRNAs and novel 3p- and 5p- derived miRNAs.
Differential expression of miRNAs based on normalized deep-sequencing counts was analyzed by selectively using, Student t test . The significance threshold was set to be 0.01 and 0.05 in each test
Genome_build: GCF_001704415.1
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| Submission date |
Nov 29, 2019 |
| Last update date |
Mar 06, 2020 |
| Contact name |
Feng Pang |
| E-mail(s) |
19871122asdf@163.com
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| Organization name |
Hainan University
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| Street address |
Renmin Road 58
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| City |
Haikou |
| ZIP/Postal code |
570228 |
| Country |
China |
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| Platform ID |
GPL19149 |
| Series (2) |
| GSE141162 |
Differentially expressed miRNAs in GSF cells infected by Orf virus at 18h.p.i (OV18h.p.i) and 30h.p.i (OV30h.p.i) compared with the mock-infected GSF cells [miRNA-seq] |
| GSE141164 |
Differentially expressed miRNAs and mRNAs in GSF cells infected by Orf virus at 18h.p.i (OV18h.p.i) and 30h.p.i (OV30h.p.i) compared with the mock-infected GSF cells |
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| Relations |
| BioSample |
SAMN13427476 |
| SRA |
SRX7241604 |
| Supplementary data files not provided |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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