|
| Status |
Public on Nov 28, 2022 |
| Title |
VOF F0 sperm-2 |
| Sample type |
SRA |
| |
|
| Source name |
VOF F0 sperm-2
|
| Organism |
Mus musculus |
| Characteristics |
cell type: sperm
strain: C57BL/6
age: 18 month old
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
The cauda epididymis was removed and sperm swam out in Tyrode's solution at 37℃ in a humidified 5% CO2 atmosphere for 15 min.And genomic sperm DNA was extracted using the Easy Pure Genomic DNA Kit (EE101, Transgen Biotech, Beijing, China).
phenol-chloroform extraction followed by bisulfite treatment,converted and fragmented genomic DNA library as described in Stroud et al., Cell 2013
|
| |
|
| Library strategy |
Bisulfite-Seq |
| Library source |
genomic |
| Library selection |
RANDOM |
| Instrument model |
HiSeq X Ten |
| |
|
| Data processing |
We applied quality and adapter trimming to FastQ files with the Perl Programm Trim Galore (version 0.4.3).
Bismark v0.16.3 mapping to reference genome
methylKit Differential methylation analysis was performed using methylKit (Version 1.4.0)
Genome_build: mm10_GRCm38
Supplementary_files_format_and_content: TXT file contain differential methylation results
|
| |
|
| Submission date |
Dec 02, 2019 |
| Last update date |
Nov 28, 2022 |
| Contact name |
Wen-Long Zhao |
| E-mail(s) |
zbyzwl1919@sina.com
|
| Organization name |
The International Peace Maternity and Child Health Hospital
|
| Street address |
Guangyuan road 145#
|
| City |
Shanghai |
| ZIP/Postal code |
200030 |
| Country |
China |
| |
|
| Platform ID |
GPL21273 |
| Series (1) |
| GSE141249 |
Whole Genome Bisulfite Sequencing Facilitates Quantitative Analysis of DNA Methylation status in sperm from 2 month old and 18 month old mice |
|
| Relations |
| BioSample |
SAMN13443065 |
| SRA |
SRX7254980 |
