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Sample GSM4199270 Query DataSets for GSM4199270
Status Public on Nov 28, 2022
Title VOF F0 sperm-2
Sample type SRA
 
Source name VOF F0 sperm-2
Organism Mus musculus
Characteristics cell type: sperm
strain: C57BL/6
age: 18 month old
Extracted molecule genomic DNA
Extraction protocol The cauda epididymis was removed and sperm swam out in Tyrode's solution at 37℃ in a humidified 5% CO2 atmosphere for 15 min.And genomic sperm DNA was extracted using the Easy Pure Genomic DNA Kit (EE101, Transgen Biotech, Beijing, China).
phenol-chloroform extraction followed by bisulfite treatment,converted and fragmented genomic DNA library as described in Stroud et al., Cell 2013
 
Library strategy Bisulfite-Seq
Library source genomic
Library selection RANDOM
Instrument model HiSeq X Ten
 
Data processing We applied quality and adapter trimming to FastQ files with the Perl Programm Trim Galore (version 0.4.3).
Bismark v0.16.3 mapping to reference genome
methylKit Differential methylation analysis was performed using methylKit (Version 1.4.0)
Genome_build: mm10_GRCm38
Supplementary_files_format_and_content: TXT file contain differential methylation results
 
Submission date Dec 02, 2019
Last update date Nov 28, 2022
Contact name Wen-Long Zhao
E-mail(s) zbyzwl1919@sina.com
Organization name The International Peace Maternity and Child Health Hospital
Street address Guangyuan road 145#
City Shanghai
ZIP/Postal code 200030
Country China
 
Platform ID GPL21273
Series (1)
GSE141249 Whole Genome Bisulfite Sequencing Facilitates Quantitative Analysis of DNA Methylation status in sperm from 2 month old and 18 month old mice
Relations
BioSample SAMN13443065
SRA SRX7254980

Supplementary file Size Download File type/resource
GSM4199270_VOF_F0_sperm-2_CpG.txt.gz 64.4 Mb (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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