|
| Status |
Public on Jun 01, 2020 |
| Title |
QMa-25 mJ |
| Sample type |
RNA |
| |
|
| Source name |
Primary melanocytes
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: melanocytes
rna fraction: exosome
treatment: irradiated
|
| Treatment protocol |
A dose of irradiation was applied to quiescent melanocytes on day 1 and then again 24 hrs later.
|
| Growth protocol |
Cultured melanocytes were made quiescent prior to irradiation (solar UVA+UVB)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
By 64 hr post-irradiation, exosomes from the irradiated or un-irradiated melanocytes were subjected to RNA extraction using the Total Exosome RNA and Protein Kit (Invitrogen).
|
| Label |
Fluorescence from FAM/SYBR green
|
| Label protocol |
n/a
|
| |
|
| Hybridization protocol |
n/a
|
| Scan protocol |
n/a
|
| Description |
irradiated
human miRNA Card A
|
| Data processing |
Raw Ct values were obtained by using Applied Biosystems TaqMan SDS v2.4 software. These values were then imported into Applied Biosystems RQ manager or Expression Suite software to quantify the relative expression of miRNAs in each of the un-irradiated and irradiated samples. Both software packages use the comparative CT method (Delta Delta CT), which translates into an RQ value.
MammU6 was used as an endogenous control, and miRNAs with a Ct value >36 or failing to amplify with an efficiency coefficient less than 1.0 were omitted from the analysis by ABI's Expression suite software.
|
| |
|
| Submission date |
Dec 06, 2019 |
| Last update date |
Jun 02, 2020 |
| Contact name |
Nikki Harter |
| E-mail(s) |
mnh5@case.edu
|
| Phone |
216-368-8693
|
| Organization name |
Case Western Reserve University
|
| Department |
Biochemistry
|
| Street address |
10900 Euclid Avenue
|
| City |
Cleveland |
| State/province |
Ohio |
| ZIP/Postal code |
44109 |
| Country |
USA |
| |
|
| Platform ID |
GPL24558 |
| Series (2) |
| GSE141619 |
Solar UVR and exosomal miRNAs effect pre-mature senescence in melanocytes [RT-PCR] |
| GSE141620 |
Solar UVR and exosomal miRNAs effect pre-mature senescence in melanocytes |
|
