|
| Status |
Public on Dec 10, 2019 |
| Title |
TST+/IGRA+ subject#1 |
| Sample type |
protein |
| |
|
| Source name |
Serum
|
| Organism |
Homo sapiens |
| Characteristics |
infection states: Asymptomatic controlled Mtb infection
|
| Treatment protocol |
Diluted 1:50
|
| Growth protocol |
N/A
|
| Extracted molecule |
protein |
| Extraction protocol |
Serum was obtained from peripheral blood samples
|
| Label |
SureLight P3 Cy5
|
| Label protocol |
SureLight®P3 Cy5
|
| |
|
| Hybridization protocol |
Arrays were blocked with 3% BSA overnight, and serum samples (diluted 1:50) was incubated on the array for 4 hours at 37 C and gentle agitation (100 RPM). Slides were then washed with PBS contain 0.05% Tween (PBST). After washing, bound antibodies were detected by goat anti-human biotin-labeled IgG (Jackson Immunoresearch), followed by a Streptavidin probe tagged with SureLight®P3 Cy5 (Cayman Chemicals). Slides were then washed with PBST, spun dry in a centrifuge, and scanned.
|
| Scan protocol |
The slides were scanned using the GenePix 4000 Microarray scanner system. Scanned images were analyzed with by the image-processing software Spotfinder (http://www.tm4.org/spotfinder.html).
|
| Description |
IgG
|
| Data processing |
The median pixel intensity of each feature was background subtracted. A minimum value of pixel intensity (400) was determined by the median pixel intensity of the spots with low quality, which was determined by the software quality control score for each spot depending on signal-to-noise ratio and spot shape. The scanned images were visually inspected. The spots with obvious local artifacts were excluded from analysis. The array features were printed in duplicates. The final data were averaged from the duplicates.
|
| |
|
| Submission date |
Dec 09, 2019 |
| Last update date |
Dec 10, 2019 |
| Contact name |
Jacqueline Achkar |
| E-mail(s) |
jacqueline.achkar@einsteinmed.org
|
| Organization name |
Albert Einstein College of Medicine
|
| Street address |
1300 Morris Park Avenue
|
| City |
Bronx |
| ZIP/Postal code |
10461 |
| Country |
USA |
| |
|
| Platform ID |
GPL27885 |
| Series (1) |
| GSE141679 |
Capsular glycan recognition provides antibody-mediated protection against tuberculosis |
|
