|
| Status |
Public on Jan 01, 2021 |
| Title |
CrebA-GFP_fkh-Gal4 input |
| Sample type |
SRA |
| |
|
| Source name |
Stage 11-16 embryos
|
| Organism |
Drosophila melanogaster |
| Characteristics |
genotype: fkh-GAL4::UAS-CrebA-GFP
antibody: none
|
| Growth protocol |
Embryos were collected for 8h at RT and allowed to age for 11 hours prior to their isloation.
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Chromatin from two independent collections of stage 11-16 embryos were crosslinked at RT in 1.8% formaldehyde and homogenized for chromatin extraction. Anti-GFP antibody or anti-CrebA antiserum were used for immunoprecipitation.
Input and immunoprecipitated DNA were prepared for sequencing using the Illumina TruSeq ChIP Sample Preparation Kit per the manufacturer's instructions. Library construction was performed by the UCLA Technology Center for Genomics & Bioinformatics.
|
| |
|
| Library strategy |
ChIP-Seq |
| Library source |
genomic |
| Library selection |
ChIP |
| Instrument model |
Illumina Genome Analyzer |
| |
|
| Description |
GFP-tagged CrebA transcription factor driven by fkh-Gal4 driver
|
| Data processing |
ChIP-seq reads were aligned to the dm6 genome assembly using BWA (Galaxy Tool Version 0.2.3) with default settings.
Replicates were merged using SamMerge (Galaxy Tool Version 1.1.2)
Peaks were called using MACS version 2.1.0.20140616 with a qvalue cutoff of 5.00e-02.
dm6
Genome_build: bed
|
| |
|
| Submission date |
Dec 10, 2019 |
| Last update date |
Jan 01, 2021 |
| Contact name |
Matthew Slattery |
| Organization name |
University of Minnesota Medical School
|
| Street address |
1035 University Drive, SMed 219
|
| City |
Duluth |
| State/province |
MINNESOTA |
| ZIP/Postal code |
55812 |
| Country |
USA |
| |
|
| Platform ID |
GPL9058 |
| Series (1) |
| GSE141778 |
Genome-wide binding of CrebA in the Drosophila salivary gland |
|
| Relations |
| BioSample |
SAMN13526956 |
| SRA |
SRX7320034 |
