|
| Status |
Public on Nov 03, 2020 |
| Title |
Deadpan_1 |
| Sample type |
RNA |
| |
|
| Source name |
3rd instar arval CNS
|
| Organism |
Drosophila melanogaster |
| Characteristics |
genotype: tubGal80ts;grhGal4/UAS-dpn
tissue: 3rd instar larval brains
|
| Treatment protocol |
24h post incubation at 30°C, wandering 3rd instar lavae were harvested
|
| Growth protocol |
Animals from the following genotype, control animals: tubGal80ts;grhGal4/UAS-cd8GFP, overexpressing activated Notch: tubGal80ts;grhGal4/UAS-NΔECD, Hes overexpressing animals: tubGal80ts;grhGal4/UAS-dpn, tubGal80ts;grhGal4/UAS-mgamma,UAS-m8, tubGal80ts;grhGal4/UAS-dpn,UAS-mgamma, were grown at restrictive temperature 18°C till late 2nd instar 8days and moved to permissive temperature 30°C for 24 hours.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was isolated from 300 larval CNSs dissected in cold PBS using the RNeasy Plus Mini Kit (Qiagen)
|
| Label |
Affymetrix array
|
| Label protocol |
Synthesis of double-stranded cDNA and biotin-labelled cRNA was performed according to manufacturer’s instructions (Affymetrix)
|
| |
|
| Hybridization protocol |
Fragmented cRNA preparations were hybridized to Drosophila genome oligonucleotide arrays (GeneChip Drosophila Genome 2.0 Array (3' IVT Expression_Affymetrix)] using an Affymetrix hybridization Oven 640, washed, and then scanned on a GeneChip Scanner 3000
|
| Scan protocol |
GeneChip Scanner 3000
|
| Description |
Gene expression data from Hes induced neural hyperplasia
|
| Data processing |
Data were analysed using Transcription activation console to generate CHP files, further Quantile normalised using R for Differential expression analysis. Trimmed mean expression intensity of >75 arbitrarily set as minimal expression
|
| |
|
| Submission date |
Dec 10, 2019 |
| Last update date |
Nov 04, 2020 |
| Contact name |
Christos Delidakis |
| E-mail(s) |
delidaki@imbb.forth.gr
|
| Phone |
+302810 391112
|
| Organization name |
Foundation for Research and Technology - Hellas
|
| Department |
Institute of Molecular Biology and Biotechnology (IMBB)
|
| Lab |
Neural development
|
| Street address |
Nikolaou Plastira 100
|
| City |
Heraklion |
| State/province |
Crete |
| ZIP/Postal code |
GR-70013 |
| Country |
Greece |
| |
|
| Platform ID |
GPL1322 |
| Series (2) |
| GSE141793 |
Hes mediated transcriptomics in Drosophila larval CNS |
| GSE141794 |
Hes protein Deadpan in neural stem cells from Drosophila 3rd instar larvae |
|
