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Sample GSM421885 Query DataSets for GSM421885
Status Public on Jul 13, 2009
Title SK-BR-7
Sample type RNA
 
Source name breast cancer cell line
Organism Homo sapiens
Characteristics cell line: SK-BR-7
cell type: breast cancer
morphology: spindle
Growth protocol Cell lines were grown to optimal cell density in RPMI 1640 supplemented with 10% FBS at 37C. Medium was changed 24h prior to RNA isolation.
Extracted molecule total RNA
Extraction protocol Cells were washed with PBS at 37C prior to isolation. Total RNA was isolated using the Rneasy kit from Qiagen. Lysis of the cells was performed in the culture flask directly after washing with PBS. Total RNA was DNAseI-treated for 1 hour at 37C. RNA QC using Agilent Technologies 2100 Bioanalyzer.
Label biotin
Label protocol Biotinylated cRNA was prepared according to the standard Affymetrix protocol from 10 ug total RNA.
 
Hybridization protocol Standard Affymetrix protocol.
Scan protocol Standard Affymetrix protocol.
Description Gene expression of SK-BR-7.
Data processing Intensity values for all genes were scaled using the global normalization factor as specified by GCOS 1.1.
 
Submission date Jun 24, 2009
Last update date Sep 01, 2016
Contact name Antoinette Hollestelle
E-mail(s) a.hollestelle@erasmusmc.nl
Phone +31-10-7044372
Fax +31-10-7044377
Organization name Erasmus University Medical Center
Department Medical Oncology
Lab Genomics and Proteomics of Breast Cancer
Street address Dr. Molenwaterplein 50
City Rotterdam
ZIP/Postal code 3015 GE
Country Netherlands
 
Platform ID GPL96
Series (1)
GSE16795 Affymetrix HG-U133A gene expression profiles of 39 human breast cancer cell lines
Relations
Reanalyzed by GSE86363

Data table header descriptions
ID_REF
VALUE GCOS1.1 signal intensity

Data table
ID_REF VALUE
1007_s_at 496.4
1053_at 343
117_at 26.1
121_at 274.9
1255_g_at 16.4
1294_at 48.4
1316_at 20.8
1320_at 26
1405_i_at 10
1431_at 6.9
1438_at 3.9
1487_at 131.4
1494_f_at 43.8
1598_g_at 320.9
160020_at 71.9
1729_at 128.9
1773_at 90.3
177_at 53.3
179_at 167.8
1861_at 148.8

Total number of rows: 22283

Table truncated, full table size 347 Kbytes.




Supplementary file Size Download File type/resource
GSM421885.CEL.gz 2.1 Mb (ftp)(http) CEL
Processed data included within Sample table

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