|
| Status |
Public on Dec 31, 2018 |
| Title |
6_1_PD1 |
| Sample type |
RNA |
| |
|
| Source name |
peripheral blood
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: CD4+ T cell
treatment protocol: 8 hrs CD3CD28 activation + PD1
|
| Treatment protocol |
Immediately after isolation or after described treatment, cells were lysed in Trizol reagent.
|
| Growth protocol |
Freshly isolated CD4+CD25low/intCD127+ T cells; CD4+ T cells were cultured as indicated. CD4+CD25+ Treg cells were expanded in cell culture.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with Trizol reagent, followed by clean-up and DNase I treatment with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with NanoDrop ThermoScientific.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
replicate 1
|
| Data processing |
The data were normalised using quantile normalisation in R
|
| |
|
| Submission date |
Jun 25, 2009 |
| Last update date |
Dec 31, 2018 |
| Contact name |
Joachim Schultze |
| E-mail(s) |
j.schultze@uni-bonn.de
|
| Organization name |
LIMES (Life and Medical Sciences Center Genomics and Immunoregulation)
|
| Department |
Genomics and Immunoregulation
|
| Street address |
Carl-Troll-Strasse 31
|
| City |
Bonn |
| State/province |
NRW |
| ZIP/Postal code |
53115 |
| Country |
Germany |
| |
|
| Platform ID |
GPL2507 |
| Series (2) |
| GSE15390 |
FOXP3-mediated inhibition of the global gene regulator SATB1 is required for maintaining regulatory T cell commitment |
| GSE16843 |
Comparison of the transcriptional profile of PD1-treated CD4+ T cells with a Treg cell phenotype and Tconv cells |
|
