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Sample GSM4226619 Query DataSets for GSM4226619
Status Public on Dec 01, 2022
Title PTZ+ Oxaprozin rep04
Sample type RNA
 
Source name adult head, 7 days of treatment
Organism Drosophila melanogaster
Characteristics tissue: head
diet fed: PTZ+ Oxaprozin
genotype: Oregon R
gender: male
age: 10 days
Stage: adult
Treatment protocol At the end of treatment, flies were anesthetized using CO2 and collected in Trizol.
Growth protocol 30 adult unmated males Oregon R flies per vial fed respective diet for 7 days at 25C temperature.
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions (RNeasy Microarray Tissue Handbook) .
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 250 ng total RNA (GeneChip 3’ IVT Express Kit).
 
Hybridization protocol Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G.
Description Gene expression data using Affymetrix Drosophila Genome 2.0 array
Data processing The data was analysed using R/Bioconductor affy and limma packages. Quantile Normalization and RMA background correction was used.
 
Submission date Dec 19, 2019
Last update date Dec 01, 2022
Contact name Manoj Ramesh Teltumbade
E-mail(s) teltumbademanoj@gmail.com
Organization name Institute of Genomics and Integrative Biology
Lab Drosophila Lab
Street address Mathura Road, Near Sukhdev Vihar, bus depo
City New Delhi
State/province Delhi
ZIP/Postal code 110025
Country India
 
Platform ID GPL1322
Series (1)
GSE142384 Drug screening in Drosophila for repurposing in neuropsychiatry

Data table header descriptions
ID_REF
VALUE log2 RMA signal

Data table
ID_REF VALUE
1616608_a_at 10.01082913
1622892_s_at 6.722358562
1622893_at 12.5457414
1622894_at 5.567168491
1622895_at 6.001065006
1622896_at 7.499743174
1622897_at 3.310825095
1622898_a_at 6.911224678
1622899_at 3.157614492
1622900_at 2.079213733
1622901_at 2.203428153
1622902_at 6.442071136
1622903_s_at 7.081217396
1622904_at 2.987491603
1622905_at 2.168622113
1622906_at 5.832669783
1622907_at 7.964140601
1622908_a_at 4.94947033
1622909_at 8.127691724
1622910_at 2.062531936

Total number of rows: 18952

Table truncated, full table size 432 Kbytes.




Supplementary file Size Download File type/resource
GSM4226619_DC19_C_Drosophila_2_.CEL.gz 1.7 Mb (ftp)(http) CEL
Processed data included within Sample table

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