|
Status |
Public on Dec 01, 2020 |
Title |
01_DMSO |
Sample type |
RNA |
|
|
Source name |
CFBE_DMSO
|
Organism |
Homo sapiens |
Characteristics |
tag: Cell line: CFBE treatment: DMSO for 24h
|
Treatment protocol |
Cells were transfected with oligonucleotides using lipofectamine RNAiMAX. Cells were treated with 6µM Corrector Compound C18 dissolved in DMSO. Cells were incubated at the noted temperatures. For detailed protocols, see PMID: 23624792.
|
Growth protocol |
CFBE cells were grown in MEM, 10% FBS, and 1µg/ml puromycin. For detailed protocols, see PMID: 23624792.
|
Extracted molecule |
total RNA |
Extraction protocol |
mirVana isolation kit was used following manufacturer's recommended protocol to extract RNA.
|
Label |
Biotin
|
Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays.
|
|
|
Hybridization protocol |
Standard Illumina hybridization protocol.
|
Scan protocol |
Standard Illumina scanning protocol.
|
Description |
DMSO treatment for 24h Replicate 1
|
Data processing |
The data were quantile normalized with background subtracted and missing data excluded using GenomeStudio and Partek Genomics Suite.
|
|
|
Submission date |
Dec 26, 2019 |
Last update date |
Dec 01, 2020 |
Contact name |
Paul B McCray |
E-mail(s) |
paul-mccray@uiowa.edu
|
Phone |
(319) 335-6844
|
Organization name |
University of Iowa
|
Department |
Pediatrics
|
Lab |
Paul B. McCray, Jr.
|
Street address |
6320 PBDB
|
City |
Iowa City |
State/province |
IA |
ZIP/Postal code |
52242 |
Country |
USA |
|
|
Platform ID |
GPL10904 |
Series (1) |
GSE142610 |
Integrative genomic meta-analysis reveals novel molecular insights into cystic fibrosis and ΔF508-CFTR rescue |
|