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Sample GSM4232834 Query DataSets for GSM4232834
Status Public on Dec 01, 2020
Title 01_DMSO
Sample type RNA
 
Source name CFBE_DMSO
Organism Homo sapiens
Characteristics tag: Cell line: CFBE
treatment: DMSO for 24h
Treatment protocol Cells were transfected with oligonucleotides using lipofectamine RNAiMAX. Cells were treated with 6µM Corrector Compound C18 dissolved in DMSO. Cells were incubated at the noted temperatures. For detailed protocols, see PMID: 23624792.
Growth protocol CFBE cells were grown in MEM, 10% FBS, and 1µg/ml puromycin. For detailed protocols, see PMID: 23624792.
Extracted molecule total RNA
Extraction protocol mirVana isolation kit was used following manufacturer's recommended protocol to extract RNA.
Label Biotin
Label protocol Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays.
 
Hybridization protocol Standard Illumina hybridization protocol.
Scan protocol Standard Illumina scanning protocol.
Description DMSO treatment for 24h
Replicate 1
Data processing The data were quantile normalized with background subtracted and missing data excluded using GenomeStudio and Partek Genomics Suite.
 
Submission date Dec 26, 2019
Last update date Dec 01, 2020
Contact name Paul B McCray
E-mail(s) paul-mccray@uiowa.edu
Phone (319) 335-6844
Organization name University of Iowa
Department Pediatrics
Lab Paul B. McCray, Jr.
Street address 6320 PBDB
City Iowa City
State/province IA
ZIP/Postal code 52242
Country USA
 
Platform ID GPL10904
Series (1)
GSE142610 Integrative genomic meta-analysis reveals novel molecular insights into cystic fibrosis and ΔF508-CFTR rescue

Data table header descriptions
ID_REF
VALUE Quantile normalized data were shifted by 24.12856 and log2 transformed.
01_DMSO.Detection Pval

Data table
ID_REF VALUE 01_DMSO.Detection Pval
7A5 7.00047 0
A1BG 4.79408 0.294553
A1CF 4.47919 0.4446157
A26C3 4.11671 0.9261602
A2BP1 4.13936 0.9125137
A2LD1 5.61975 0.003896104
A2M 4.18512 0.5922078
A2ML1 4.53153 0.5311688
A3GALT2 4.208 0.6892824
A4GALT 5.73702 0.003896104
A4GNT 5.74065 0.006493506
AAA1 4.28554 0.9282225
AAAS 5.77071 0.002597403
AACS 6.76254 0
AACSL 4.95873 0.1779221
AADAC 4.62162 0.4077922
AADACL1 9.04356 0
AADACL2 4.57784 0.4051948
AADACL3 4.64879 0.3844156
AADACL4 5.31008 0.01428571

Total number of rows: 34602

Table truncated, full table size 817 Kbytes.




Supplementary data files not provided
Processed data included within Sample table

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