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Status |
Public on Dec 28, 2019 |
Title |
shoot apical, 3798c rep1 |
Sample type |
SRA |
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Source name |
stem tip
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Organism |
Gossypium hirsutum |
Characteristics |
strain: 3798 tissue: shoot apical developmental stage: seedling stage genotype: control phenotype: nulliplex-branch
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Extracted molecule |
total RNA |
Extraction protocol |
RNA was harvested using Plant RNA EASYspin Plus Kit(Aidlab, Peking, China). RNA libraries were prepared for sequencing using standard Illumina protocols
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Library strategy |
RNA-Seq |
Library source |
transcriptomic |
Library selection |
cDNA |
Instrument model |
Illumina HiSeq 2000 |
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Description |
T01
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Data processing |
Illumina Casava1.7 software used for basecalling. Reads were aligned to cotton genome no more than 5 mismatches using the SOAP aligner/SOAP2 software after low-quality reads (reads with unknown sequences ‘N’), adaptor sequence fragments, and empty reads were removed Reads Per Kilobase of exon per Megabase of library size (RPKM) were calculated using a protocol from Chepelev et al., Nucleic Acids Research, 2009. Genome_build: NCBI WGS Project: LBLM01 (Gossypium hirsutum) Supplementary_files_format_and_content: tab-delimited text file includes FPKM values for each Sample
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Submission date |
Dec 27, 2019 |
Last update date |
Dec 30, 2019 |
Contact name |
quan sun |
E-mail(s) |
sunquan@cqupt.edu.cn
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Organization name |
Chongqing University of Posts and Telecommunications
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Department |
College of Bioinformation
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Street address |
Chongwen road 2#
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City |
Chongqing |
ZIP/Postal code |
400065 |
Country |
China |
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Platform ID |
GPL16485 |
Series (1) |
GSE142638 |
mRNA Transcriptase’s Analyses That Cotton fruit branch Development Status after silenced GhBRC1 gene byVIGS |
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Relations |
BioSample |
SAMN13684530 |
SRA |
SRX7444785 |
Supplementary data files not provided |
SRA Run Selector |
Raw data are available in SRA |
Processed data are available on Series record |
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