|
| Status |
Public on Jan 04, 2020 |
| Title |
85A10_48h_ORN (N543Barcode_715-508) |
| Sample type |
SRA |
| |
|
| Source name |
in house
|
| Organism |
Drosophila melanogaster |
| Characteristics |
genotype: 85A10-GAL4
number of cells: 1
orn type: labels 5 ORN types
|
| Treatment protocol |
None
|
| Growth protocol |
None
|
| Extracted molecule |
total RNA |
| Extraction protocol |
None
Modified SMART-seq2 (Li et al. 2017)
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
RACE |
| Instrument model |
NextSeq 550 |
| |
|
| Description |
Single cell
df_libs.csv, htseq.tab
|
| Data processing |
library strategy: 5’ RACE RNA-seq
alignment (STAR 2.4.2a, ENCODE standard options, except --outFilterScoreMinOverLread 0.4 --outFilterMatchNminOverLread 0.4 -- outFilterMismatchNmax 999 --outFilterMismatchNoverLmax 0.04)
gene count (HTSeq-count 0.7.1, default options, except -m intersection-strict)
Genome_build: Dmel r6.10
Supplementary_files_format_and_content: df_htseq_ORN.csv, df_libs_ORN.csv
|
| |
|
| Submission date |
Jan 03, 2020 |
| Last update date |
Jan 04, 2020 |
| Contact name |
Hongjie Li |
| E-mail(s) |
hongjie.li@bcm.edu
|
| Organization name |
Baylor College of Medicine
|
| Street address |
one Baylor Plaza
|
| City |
Houston |
| State/province |
TX |
| ZIP/Postal code |
77030 |
| Country |
USA |
| |
|
| Platform ID |
GPL22106 |
| Series (1) |
| GSE143038 |
Single-cell Transcriptomes Reveal Diverse Regulatory Strategies for Olfactory Receptor Expression and Axon Targeting |
|
| Relations |
| BioSample |
SAMN13719949 |
| SRA |
SRX7498872 |
