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Status |
Public on Mar 21, 2012 |
Title |
C2C12_96hr_differentiation_rep3 |
Sample type |
RNA |
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Source name |
C2C12 myoblasts, differentiating, 96h
|
Organism |
Mus musculus |
Characteristics |
cell line: C2C12 time point: 96hr_differentiation replicate: 3
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Treatment protocol |
For cycloheximide-treated samples, cells were pre-treated with 50uM cycloheximide 1 hour prior to induction of differentiation.
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Growth protocol |
C2C12 myoblasts were cultured in DMEM + 20% FBS before inducing differentiation by changing to DMEM + 2% horse serum.
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Extracted molecule |
total RNA |
Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
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Label |
biotin
|
Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
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Hybridization protocol |
Following fragmentation, 20 ug of cRNA were hybridized using standard conditions to Affymetrix MOE-430v2 expression arrays. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
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Scan protocol |
GeneChips were scanned using the Affymetrix Gene Chip 3000 scanner.
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Description |
Gene expression data from differentiating C2C12 myoblasts grown in 2% horse serum
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Data processing |
Raw data was processed using the FlexArray package. Data was normalized using RMA with background correction.
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Submission date |
Jul 08, 2009 |
Last update date |
Mar 21, 2012 |
Contact name |
Saravanan Sundararajan |
Organization name |
McGill University
|
Department |
Human Genetics
|
Lab |
Rob Sladek
|
Street address |
740, Dr Penfield Ave
|
City |
Montreal |
State/province |
Quebec |
ZIP/Postal code |
H3A 1A4 |
Country |
Canada |
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|
Platform ID |
GPL1261 |
Series (2) |
GSE16992 |
Expression Profiling of Early Myogenesis - Affymetrix Dataset |
GSE17039 |
Expression Profiling of Early Myogenesis |
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