|
| Status |
Public on Mar 21, 2012 |
| Title |
C2C12_3hr_CHX_rep3 |
| Sample type |
RNA |
| |
|
| Source name |
C2C12 myoblasts, differentiating, CHX pre-treated, 3h
|
| Organism |
Mus musculus |
| Characteristics |
cell line: C2C12
time point: 3hr_CHX
replicate: 3
|
| Treatment protocol |
For cycloheximide-treated samples, cells were pre-treated with 50uM cycloheximide 1 hour prior to induction of differentiation.
|
| Growth protocol |
C2C12 myoblasts were cultured in DMEM + 20% FBS before inducing differentiation by changing to DMEM + 2% horse serum.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 20 ug of cRNA were hybridized using standard conditions to Affymetrix MOE-430v2 expression arrays. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix Gene Chip 3000 scanner.
|
| Description |
Gene expression data from differentiating C2C12 myoblasts pre-treated with 50uM cycloheximide
|
| Data processing |
Raw data was processed using the FlexArray package. Data was normalized using RMA with background correction.
|
| |
|
| Submission date |
Jul 08, 2009 |
| Last update date |
Mar 21, 2012 |
| Contact name |
Saravanan Sundararajan |
| Organization name |
McGill University
|
| Department |
Human Genetics
|
| Lab |
Rob Sladek
|
| Street address |
740, Dr Penfield Ave
|
| City |
Montreal |
| State/province |
Quebec |
| ZIP/Postal code |
H3A 1A4 |
| Country |
Canada |
| |
|
| Platform ID |
GPL1261 |
| Series (2) |
| GSE16992 |
Expression Profiling of Early Myogenesis - Affymetrix Dataset |
| GSE17039 |
Expression Profiling of Early Myogenesis |
|
