|
| Status |
Public on Feb 10, 2010 |
| Title |
Adult male, Hemiclone line P15, Repl. 3 |
| Sample type |
RNA |
| |
|
| Source name |
Adult male, Hemiclone line P15
|
| Organism |
Drosophila melanogaster |
| Characteristics |
tissue: whole fly
genotype: LHm, hemiclone line
age: Day 14 (4 days adult)
|
| Treatment protocol |
Adult hemiclonal and LHM tester flies of both sexes (reared following the base population protocol) were then collected in groups of 16 on day-10. On day-12 each group of hemiclones was placed together with a group of tester flies of the opposite sex in yeasted vials. After 24 hours the tester flies were removed and after a further 20 hours groups of 6 flies were randomly chosen from each vial under brief CO2 anaesthesia. Four hours after sorting, the flies were frozen using liquid nitrogen prior to RNA extraction.
|
| Growth protocol |
The base population of Drosophila melanogaster (LHM) has been maintained as a large, outbred population for over 400 non-overlapping generations. One hundred haplotypes were sampled from LHM and maintained as heterozygous stock hemiclonal lines using double-X clone-generator females [C(1)DX, y, f; T(2;3) rdgC st in ri pP bwD]. Each hemiclonal fly therefore shares one complete genomic haplotype in common, the other being a random sample from the base population.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions, followed by Qiagen purification.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
| Description |
P15M3
|
| Data processing |
The data were analyzed with RMA (Robust Multichip Average) as implemented by the affy package in R 2.9 (BioConductor 2.4) using default settings.
|
| |
|
| Submission date |
Jul 08, 2009 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Paolo Innocenti |
| E-mail(s) |
paolo.innocenti@ebc.uu.se
|
| Organization name |
Uppsala university
|
| Department |
Dept. of Animal Ecology
|
| Lab |
Morrow Lab
|
| Street address |
Norbyvägen 18D
|
| City |
Uppsala |
| ZIP/Postal code |
75236 |
| Country |
Sweden |
| |
|
| Platform ID |
GPL1322 |
| Series (1) |
| GSE17013 |
The sexually antagonistic genes of Drosophila melanogaster |
|
| Relations |
| Reanalyzed by |
GSE119084 |
