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Sample GSM425603 Query DataSets for GSM425603
Status Public on Feb 10, 2010
Title Adult female, Hemiclone line P18, Repl. 4
Sample type RNA
 
Source name Adult female, Hemiclone line P18
Organism Drosophila melanogaster
Characteristics tissue: whole fly
genotype: LHm, hemiclone line
age: Day 14 (4 days adult)
Treatment protocol Adult hemiclonal and LHM tester flies of both sexes (reared following the base population protocol) were then collected in groups of 16 on day-10. On day-12 each group of hemiclones was placed together with a group of tester flies of the opposite sex in yeasted vials. After 24 hours the tester flies were removed and after a further 20 hours groups of 6 flies were randomly chosen from each vial under brief CO2 anaesthesia. Four hours after sorting, the flies were frozen using liquid nitrogen prior to RNA extraction.
Growth protocol The base population of Drosophila melanogaster (LHM) has been maintained as a large, outbred population for over 400 non-overlapping generations. One hundred haplotypes were sampled from LHM and maintained as heterozygous stock hemiclonal lines using double-X clone-generator females [C(1)DX, y, f; T(2;3) rdgC st in ri pP bwD]. Each hemiclonal fly therefore shares one complete genomic haplotype in common, the other being a random sample from the base population.
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions, followed by Qiagen purification.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
 
Hybridization protocol Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Scan protocol GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
Description P18F4
Data processing The data were analyzed with RMA (Robust Multichip Average) as implemented by the affy package in R 2.9 (BioConductor 2.4) using default settings.
 
Submission date Jul 08, 2009
Last update date Aug 28, 2018
Contact name Paolo Innocenti
E-mail(s) paolo.innocenti@ebc.uu.se
Organization name Uppsala university
Department Dept. of Animal Ecology
Lab Morrow Lab
Street address Norbyvägen 18D
City Uppsala
ZIP/Postal code 75236
Country Sweden
 
Platform ID GPL1322
Series (1)
GSE17013 The sexually antagonistic genes of Drosophila melanogaster
Relations
Reanalyzed by GSE119084

Data table header descriptions
ID_REF
VALUE RMA standard.

Data table
ID_REF VALUE
1616608_a_at 11.35137854
1622892_s_at 9.021576017
1622893_at 11.2607829
1622894_at 7.726934254
1622895_at 9.307501267
1622896_at 7.635017698
1622897_at 5.086703355
1622898_a_at 10.51727661
1622899_at 4.874819023
1622900_at 3.681147814
1622901_at 6.026511299
1622902_at 7.195364153
1622903_s_at 9.408986479
1622904_at 5.137713873
1622905_at 3.588673546
1622906_at 8.798199653
1622907_at 8.613552378
1622908_a_at 7.131733453
1622909_at 11.01791831
1622910_at 3.776844837

Total number of rows: 18952

Table truncated, full table size 432 Kbytes.




Supplementary file Size Download File type/resource
GSM425603.CEL.gz 2.0 Mb (ftp)(http) CEL
Processed data included within Sample table

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