|
Status |
Public on Dec 31, 2020 |
Title |
Female TCDD 1000 ng/kg rep7 |
Sample type |
RNA |
|
|
Source name |
liver_female_TCDD treated F0_1000 ng/kg
|
Organism |
Rattus norvegicus |
Characteristics |
strain: Sprague-Dawley Sex: female lineage (f0 dam, f1 male, f2 male, f3): 71B-7-5-3 tcdd dose (f0): 1000 ng/kg tissue: liver
|
Treatment protocol |
Pregnant F0 dams were treated by oral gavage with a single bolus of either TCDD (30, 100, 300 or 1000 ng/kg) dissolved in corn oil vehicle or vehicle alone (5mL/kg) on gestational day 11.
|
Growth protocol |
Upon weaning, same-sex littermates were housed in stainless steel, wire-mesh bottom cages with pelleted rat feed (R36, Lactamin, Stockholm, Sweden) and tap water available ad libitum. The housing environment was maintained at 21±0.2°C and relative humidity at 50±3%, with a 12/12h artificial light/dark cycle.
|
Extracted molecule |
total RNA |
Extraction protocol |
Total hepatic RNA was isolated for analysis on microarrays. Briefly, 20 mg hepatic tissue was ground to a powder in liquid nitrogen using a mortar and pestle, followed by rapid homogenization using a Brinkmann Polytron (Polytron PT1600E with a PT-DA 1607 generator). RNA was extracted using an RNeasy Mini Plus Kit following the manufacturer’s recommended protocol (Qiagen, Mississauga, Canada).
|
Label |
biotin
|
Label protocol |
Manufacturer's recommended protocols as followed by the TCAG Facility at the Hospital for Sick Kid's in Toronto, Canada
|
|
|
Hybridization protocol |
Manufacturer's recommended protocols as followed by the TCAG Facility at the Hospital for Sick Kid's in Toronto, Canada
|
Scan protocol |
Manufacturer's recommended protocols as followed by the TCAG Facility at the Hospital for Sick Kid's in Toronto, Canada
|
Description |
PB57
|
Data processing |
Raw array data (CEL files) were loaded into the R statistical environment using the affy package (v1.48.0) of the BioConductor library. Data was investigated for distributional homogeneity, then pre-processed using the RMA algorithm. The custom Probe to Entrez Gene ID map, ragene20strnentrezgcdf (v21.0.0) was used for annotation.
|
|
|
Submission date |
Jan 09, 2020 |
Last update date |
Jan 01, 2021 |
Contact name |
Stephenie Prokopec |
E-mail(s) |
stephenie.prokopec@oicr.on.ca
|
Organization name |
Ontario Institute for Cancer Research
|
Street address |
661 University Avenue, Suite 510
|
City |
Toronto |
State/province |
Ontario |
ZIP/Postal code |
M5G 0A3 |
Country |
Canada |
|
|
Platform ID |
GPL26164 |
Series (2) |
GSE143368 |
Transgenerational epigenetic and transcriptomic effects of 2,3,7,8-tetrachlorodibenzo-p-dioxin exposure in rat [array] |
GSE143884 |
Transgenerational epigenetic and transcriptomic effects of 2,3,7,8-tetrachlorodibenzo-p-dioxin exposure in rat |
|