|
| Status |
Public on Sep 25, 2020 |
| Title |
Tumor-127 |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
Adrenocortical carcinoma biopsy
|
| Organism |
Homo sapiens |
| Characteristics |
condition: Adrenocortical carcinoma
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted from 50-100 mg of tumor using the Qiagen miRNeasy Kit.
|
| Label |
Hy3
|
| Label protocol |
450 ng total RNA from sample and a common reference pool was labelled with Hy3™ and Hy5™ fluorescent label, respectively, using the miRCURY™ LNA Array power labelling kit (Exiqon, Denmark) following the procedure described by the manufacturer.
|
| |
|
| Channel 2 |
| Source name |
Common reference pool
|
| Organism |
Homo sapiens |
| Characteristics |
sample type: common reference
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted from 50-100 mg of tumor using the Qiagen miRNeasy Kit.
|
| Label |
Hy5
|
| Label protocol |
450 ng total RNA from sample and a common reference pool was labelled with Hy3™ and Hy5™ fluorescent label, respectively, using the miRCURY™ LNA Array power labelling kit (Exiqon, Denmark) following the procedure described by the manufacturer.
|
| |
|
| |
| Hybridization protocol |
The labeled RNA samples were hybridized to the LNA-enhanced miRCURY™ microarray version 11.0 (Exiqon, Denmark), which contained capture probes targeting all human miRNAs registered in miRBASE version 13.0 at the Sanger Institute at the time of the analysis. The hybridization was performed according to the miRCURY™ LNA array manual using a Tecan HS4800 hybridization station (Tecan, Austria).
|
| Scan protocol |
After hybridization, washing and drying, the microarray slides were scanned in an Agilent G2565BA Microarray Scanner System (Agilent Technologies, Inc., USA) and the resulting images were quantified by ImaGene v.8.0 software (BioDiscovery, Inc., USA).
|
| Data processing |
The quantified signals were background corrected (Normexp with offset value 10)3 and normalized using the global LOWESS (LOcally WEighted Scatterplot Smoothing) regression algorithm.
|
| |
|
| Submission date |
Jan 09, 2020 |
| Last update date |
Sep 25, 2020 |
| Contact name |
Thomas Litman |
| E-mail(s) |
tlitman@hotmail.com
|
| Phone |
4541851753
|
| Organization name |
University of Copenhagen
|
| Department |
ISIM
|
| Street address |
Blegdamsvej 3C
|
| City |
Copenhagen N |
| State/province |
Outside the US or Canada |
| ZIP/Postal code |
2200 |
| Country |
Denmark |
| |
|
| Platform ID |
GPL27982 |
| Series (1) |
| GSE143385 |
microRNA expression analysis of metastatic adrenocortical tumors |
|
