|
| Status |
Public on Jul 30, 2020 |
| Title |
RNAseq_Ad_lib_WT_ZT10_rep2 |
| Sample type |
SRA |
| |
|
| Source name |
Liver
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6J
gender: male
diet: normal chow
genotype/variation: WT
tissue: Liver
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from liver tissue or isolated endothelial cells and kupffer cells using Trizol reagent followed by RNeasy Mini Kit (#74104).
Libraries were prepared according to Illumina's instructions accompanying the TruSeq RNA Sample Preparation. The purified cDNA was captured on an Illumina flow cell for cluster generation. Libraries were sequenced on the Genome Analyzer following the manufacturer's protocols.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2500 |
| |
|
| Description |
Ad_lib_feeding
|
| Data processing |
RNA-seq reads were aligned to the University of California, Santa Cruz (UCSC) mm9 genome browser using STAR. The tag directories were established and normalized read counts were measured with RefSeq genes using Homer.
Genome_build: mm9
|
| |
|
| Submission date |
Jan 13, 2020 |
| Last update date |
Jul 30, 2020 |
| Contact name |
Dongyin Guan |
| E-mail(s) |
dongyin.guan@bcm.edu
|
| Organization name |
Baylor College of Medicine
|
| Lab |
Dongyin Guan
|
| Street address |
1 Baylor Plaza, ABBR
|
| City |
Houston |
| State/province |
TX |
| ZIP/Postal code |
77030 |
| Country |
USA |
| |
|
| Platform ID |
GPL17021 |
| Series (2) |
| GSE143524 |
The Hepatocyte Clock and Feeding Interdependently Control Chrono-Homeostasis of Multiple Liver Cell Types (RNA-seq) |
| GSE143528 |
The hepatocyte clock and feeding control chronophysiology of multiple liver cell types |
|
| Relations |
| BioSample |
SAMN13836333 |
| SRA |
SRX7546484 |
