 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on Jan 01, 2023 |
| Title |
Ovaries, L2 |
| Sample type |
SRA |
| |
|
| Source name |
ovarian tissue
|
| Organism |
Anas platyrhynchos |
| Characteristics |
tissue: ovarian tissue
age: week 71
phenotype: low egg number
|
| Extracted molecule |
total RNA |
| Extraction protocol |
ovarian tissue were removed, flash frozen on dry ice, and RNA was harvested using Trizol reagent. TRIzol RNA Isolation Reagents was used with 1 ug of total RNA for the construction of sequencing libraries.
RNA libraries were prepared for sequencing using standard Illumina protocols
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2500 |
| |
|
| Data processing |
Each library was indexed in order to multiplex four samples per sequencing run on the Illumina HiSeq 2500 platform.
A minimum of 6Gb 150bp sequencing reads were collected from each sample and data were analyzed using Genesifter software.
Raw data for each sample were aligned to the most recent Anas Platyrhynchos genome build in order to identify previously unknown regions that may encode for unique mRNAs.
Pairwise comparison of the alignment results was done using Genesifter for identification of mRNAs that are differentially expressed at a significant level, i.e. up- or down-regulated.
Genome_build: CAU_duck1.0 (GCA_002743455.1)
Supplementary_files_format_and_content: FPKMs.txt
|
| |
|
| Submission date |
Jan 14, 2020 |
| Last update date |
Jan 01, 2023 |
| Contact name |
Yanfa Sun |
| E-mail(s) |
boysun2010@163.com
|
| Organization name |
Longyan University
|
| Department |
College of Life Science
|
| Street address |
Dongxiao North Road No. 1, Xinluo District
|
| City |
Longyan |
| State/province |
Fujian |
| ZIP/Postal code |
364012 |
| Country |
China |
| |
|
| Platform ID |
GPL22591 |
| Series (1) |
| GSE143651 |
Regulation of actin cytoskeleton signaling pathways improves egg-production through up-regulation of integrin beta family genes expression in ducks |
|
| Relations |
| BioSample |
SAMN13853741 |
| SRA |
SRX7554596 |
| Supplementary data files not provided |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
|
|
|
|
 |
