|
| Status |
Public on Jan 21, 2020 |
| Title |
left ventricular_Exercise rats_rep1 |
| Sample type |
RNA |
| |
|
| Source name |
left ventricular_Exercise rats
|
| Organism |
Rattus norvegicus |
| Characteristics |
strain: Wistar
Sex: female
treatment: Exercise
tissue: left ventricular
|
| Treatment protocol |
For 10 weeks, from Monday to Friday, the rats in the EX group completed a 1-h swimming exercise schedule. The exercise training was performed by placing the rats in a swimming pool (150 cm × 60 cm × 70 cm) filled with warm water to a depth of 60 cm. The pool was divided by plastic barriers into eight lanes. The water temperature was maintained at 31 ± 1°C.
|
| Growth protocol |
All rats (200 ± 20 g, n = 32) were fed a standard diet, exposed to a 12-h light–12-h dark cycle, and maintained in a constant room temperature (22 ± 2°C) and humidity (50 ± 10%) .
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated using TRIzol (Invitrogen) and purified with RNeasy mini kit (QIAGEN) according to manufacturer’s instructions. RNA quality and quantity was measured by using nanodrop spectrophotometer (ND-1000, Nanodrop Technologies) and RNA Integrity was determined by gel electrophoresis.
|
| Label |
Hy3
|
| Label protocol |
The miRCURY™ Hy3™/Hy5™ Power labeling kit (Exiqon, Vedbaek, Denmark) was used according to the manufacturer’s guideline for miRNA labelling.
|
| |
|
| Hybridization protocol |
The Hy3™-labeled samples were hybridized on the miRCURYTM LNA Array (v.19.0) (Exiqon) according to array manual.
|
| Scan protocol |
The slides were scanned using the Axon GenePix 4000B microarray scanner (Axon Instruments, Foster City, CA).
|
| Description |
physiological left ventricular hypertrophy
Experimental animals in Ex group were female and commercially-available Wistar rats (SLAC, Shanghai, China). For 10 weeks, from Monday to Friday, the rats in the EX group completed a 1-h swimming exercise schedule.
|
| Data processing |
Scanned images were then imported into GenePix Pro 6.0 software (Axon) for grid alignment and data extraction. Replicated miRNAs were averaged and miRNAs that intensities>=30 in all samples were chosen for calculating normalization factor. Expressed data were normalized using the Median normalization. After normalization, significant differentially expressed miRNAs between two groups were identified through Fold change and P-value. Differentially expressed miRNAs between two samples were filtered through Fold change.
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| |
|
| Submission date |
Jan 20, 2020 |
| Last update date |
Jan 21, 2020 |
| Contact name |
Jun Zhang |
| E-mail(s) |
zhangjyzh@sina.com
|
| Organization name |
Shanghai Normal University
|
| Street address |
No.100 of Guilin Road
|
| City |
Shanghai |
| ZIP/Postal code |
200234 |
| Country |
China |
| |
|
| Platform ID |
GPL28018 |
| Series (1) |
| GSE143935 |
miRNA expression profiling in the physiological cardiac hypertrophy of rats |
|
