|
| Status |
Public on Jan 24, 2020 |
| Title |
NC-20-B |
| Sample type |
SRA |
| |
|
| Source name |
whole-organism
|
| Organism |
Nematostella vectensis |
| Characteristics |
developmental stage: adult
geographic location: USA: North Carolina
temperature: 20 C
|
| Treatment protocol |
MA animals were exposed to 20C or 30C; ME animals were exposed to 20C, 28C, or 30C; NC animals were exposed to 20C , 30C or 32C; NS animals were exposed to 20C, 28C, or 30C; SC animals were exposed to 20C, 30C or 32C. Sample sizes: nTotal=52; nMA = 8; nME = 10; nNC = 11; nNS = 11; nSC = 12
|
| Growth protocol |
Animals were maintained as described in Hand et al. (1994)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted from whole organisms using Ambion RNAqueous extraction kit.
Libraries were prepared as in Meyer et al. (2011)
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2500 |
| |
|
| Description |
genome Putnam et al. (2007)
North Carolina, 20 C, replicate B
S60
|
| Data processing |
Sequenced reads were concatenated and renamed based on sample ID
Each Illumina read was trimmed of the adapter and PCR duplicates were removed
Quality filtering was performed using fastx_toolkit
Transcriptome mapping was performed using Bowtie2
Genome_build: https://figshare.com/articles/Nematostella_vectensis_transcriptome_and_gene_models_v2_0/807696
Supplementary_files_format_and_content: tab-delimited table of counts per gene for each sample
|
| |
|
| Submission date |
Jan 23, 2020 |
| Last update date |
Jan 26, 2020 |
| Contact name |
Whitney at Leach |
| Organization name |
University of North Carolina at Charlotte
|
| Department |
Biological Sciences
|
| Lab |
Reitzel
|
| Street address |
9201 University City Blvd.
|
| City |
Charlotte |
| ZIP/Postal code |
28223 |
| Country |
USA |
| |
|
| Platform ID |
GPL23802 |
| Series (1) |
| GSE144104 |
Geographical temperature stress in the sea anemone Nematostella vectensis |
|
| Relations |
| BioSample |
SAMN13908041 |
| SRA |
SRX7622517 |
