|
| Status |
Public on Jul 17, 2009 |
| Title |
PQS treated mvfR mutant constitutively expressing PqsE from a plasmid rep1 |
| Sample type |
RNA |
| |
|
| Source name |
Pseudomonas aeruginosa PA14 mvfR mutant constitutively expressed PqsE
|
| Organism |
Pseudomonas aeruginosa |
| Characteristics |
bacteria: Pseudomonas aeruginosa
strain: PA14
mutant: mvfR
plasmid: pDN19pqsE, tetracycline resistant
compound: PQS (20ug/ml)
|
| Treatment protocol |
PQS was added to the LB at 20ug/ml.
|
| Growth protocol |
Cells were inoculated from -80C stock, grown in LB with tetracycline (120ug/ml) and PQS (20ug/ml) at 37C over night, diluted 1:100 and grown to OD600nm of 3.0
|
| Extracted molecule |
total RNA |
| Extraction protocol |
According to Qiagen RNAeasy mini kit
|
| Label |
biotin
|
| Label protocol |
According to Enzo Life sciences BioArray terminal labeling kit with biotin ddUTP.
|
| |
|
| Hybridization protocol |
According to Affymetrix GeneChip® Fluidics Station 450 protocol.
|
| Scan protocol |
According to Affymetrix scanner 3000 protocol
|
| Description |
The effect of PQS at concentration of 20ug/ml was tested on a PA14 mvfR mutant that was transformed with the plasmid pDN19pqsE containing a constitutive expressed PqsE under ptac promoter.
|
| Data processing |
Affymetrix Microarray Suite version 5.0
|
| |
|
| Submission date |
Jul 15, 2009 |
| Last update date |
Jul 16, 2009 |
| Contact name |
Laurence G Rahme |
| E-mail(s) |
rahme@molbio.mgh.harvard.edu
|
| Phone |
617-724-5003
|
| Fax |
617-724-8558
|
| Organization name |
Massachusetts General Hospital
|
| Department |
Surgery
|
| Street address |
50 Blosson St, Thier 340
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02114 |
| Country |
USA |
| |
|
| Platform ID |
GPL84 |
| Series (2) |
| GSE17116 |
Pseudomonas aeruginosa PA14, pqsE and PQS |
| GSE17147 |
Pseudomonas aeruginosa PA14 MvfR-dependent QS regulatory pathway |
|
