|
| Status |
Public on Sep 30, 2020 |
| Title |
WT_Rep2 |
| Sample type |
SRA |
| |
|
| Source name |
mature panicle
|
| Organism |
Oryza sativa Indica Group |
| Characteristics |
cultivar: Pusa Basmati 1 (PB1)
tissue: mature panicle
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA mature panicle before anthesis (Stage 12) of both wild-type, over-expression and transgenic knock-down lines of AGO17 were extracted using Trizol (Invitrogen) reagent.
Size fractionation
|
| |
|
| Library strategy |
ncRNA-Seq |
| Library source |
transcriptomic |
| Library selection |
size fractionation |
| Instrument model |
Illumina HiSeq 2500 |
| |
|
| Data processing |
3’ adapter (5’-TGGAATTCTCGGGTGCCAAGG-3’) was removed from sequenced reads using UEA small RNA workbench.
The small RNA reads of length 16nt-35nt filtered and were analyzed.
Supplementary_files_format_and_content: Text file containing 16nt-35nt long unique reads and their raw abundance after removal of adapter.
|
| |
|
| Submission date |
Jan 23, 2020 |
| Last update date |
Oct 01, 2020 |
| Contact name |
Shivaprasad Padubidri V. |
| E-mail(s) |
shivaprasad@ncbs.res.in
|
| Phone |
+91 80 2366 6511
|
| Organization name |
National Center for Biological Sciences
|
| Department |
Plant epigenetics
|
| Street address |
GKVK Campus, Bellary road
|
| City |
Bangalore |
| ZIP/Postal code |
560065 |
| Country |
India |
| |
|
| Platform ID |
GPL21027 |
| Series (1) |
| GSE144110 |
Role of rice AGO17 in rice development |
|
| Relations |
| BioSample |
SAMN13908206 |
| SRA |
SRX7622735 |
