|
| Status |
Public on Aug 11, 2020 |
| Title |
Exp3.HSC1 |
| Sample type |
SRA |
| |
|
| Source name |
HSC
|
| Organism |
Mus musculus |
| Characteristics |
strain background: C57BL/6
strain: PolyloxExpress mice
age: between 7 and 20 weeks after birth
tissue: Bone marrow
experiment: exp 3
cell type: Hematopoietic stem cells
replicate: sample 1
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Single cells were captured using the 10x Chromium system and further processed following the Chromium Single Cell 3’ Reagent Kit (v3) protocol.
cDNA library were fragmented and the sequencing library was generated according to the Single Cell 3’ Reagent Kit (v3) protocol.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 4000 |
| |
|
| Data processing |
Illumina HD.3.4.0/RTA 2.7.7 software used for sequencing and basecalling.
10x Genomics Cell Ranger v3.1.0 used for sequencing read alignment and UMI counting.
Genome_build: mm10
Supplementary_files_format_and_content: mtx, tsv: UMI counts of individual genes in single cells
|
| |
|
| Submission date |
Jan 27, 2020 |
| Last update date |
Aug 11, 2020 |
| Contact name |
Xi Wang |
| Organization name |
Nanjing Medical University
|
| Street address |
Longmian Avenue 101, Jiangning District
|
| City |
Nanjing |
| ZIP/Postal code |
21116 |
| Country |
China |
| |
|
| Platform ID |
GPL21103 |
| Series (2) |
| GSE144273 |
Resolving fate and transcriptome of hematopoietic stem cell clones [multiple cell types] |
| GSE152557 |
Resolving fate and transcriptome of hematopoietic stem cell clones |
|
| Relations |
| BioSample |
SAMN13927247 |
| SRA |
SRX7635009 |
