|
| Status |
Public on Jan 29, 2023 |
| Title |
HS578T cells - triple negative- metaplastic |
| Sample type |
RNA |
| |
|
| Source name |
RNA isolated from cells in culture
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: breast cancer
cell line: HS578T cells
phenotype: triple negative- metaplastic
|
| Growth protocol |
HS578T cells were grown in low-glucose DMEM containing 10% fetal calf serum, glutamine and insulin. BAS cells were grown in keratynocyte serum-free medium supplemented with human recombinant epidermal growth factor and bovine pituitary extract. MCF-7 and MDA-MB-231 cells were grown in low-glucose DMEM containing 10% fetal calf serum and glutamine. HMEC (primary human mammary epithelial cells; a pool from 3 isolates from normal reduction mammoplasties) and MCF-10A were grown in serum-free Mammary Epithelial Cell Growth Medium.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Three biological replicates were used to isolate miRs with the miRCURY RNA isolation kit - Exiqon. RNA was pooled and labelled.
|
| Label |
Biotin
|
| Label protocol |
Affymetrix FlashTag Biotin HSR RNA Labeling Kit
|
| |
|
| Hybridization protocol |
A single GeneChip® Cartridge Array was used per cell line. GeneChips were washed and stained in the GeneChip® Fluidics Station 450 (Affymetrix).
|
| Scan protocol |
GeneChips were scanned using the GeneChip® Scanner 3000 7G.
|
| Description |
HS-578T
|
| Data processing |
RMA-normalized expression was calculated using the R tool ‘oligo’ (Carvalho BS, Irizarry RA. “A Framework for Oligonucleotide Microarray Preprocessing” Bioinformatics (2010) 26(19), 2363-7).
|
| |
|
| Submission date |
Jan 29, 2020 |
| Last update date |
Jan 29, 2023 |
| Contact name |
Ernesto Yague |
| E-mail(s) |
ernesto.yague@imperial.ac.uk
|
| Organization name |
Imperial College London
|
| Department |
Division of Cancer
|
| Street address |
Du Cane Road
|
| City |
London |
| ZIP/Postal code |
W12 0NN |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL19117 |
| Series (1) |
| GSE144445 |
MicroRNA profile of drug resistant metaplastic breast cancer cell lines |
|
